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Induction of sister chromatid exchange in preimplantation mouse embryos in vitro by /sup 3/H-thymidine or ultraviolet light in combination with caffeine

Journal Article · · Teratog., Carcinog., Mutagen.; (United States)
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Preimplantation mouse embryos were exposed in vitro to /sup 3/H-thymidine (25, 100, or 250 Bq/ml) or ultraviolet (UV) light (1.35 or 4.05 J/m2), either alone or in combination with caffeine (1 mM with /sup 3/H-thymidine and 0.5 mM with UV light). Exposure to /sup 3/H-thymidine lasted for 2 days, from the two-cell stage to the late morula/early blastocyst stage, and UV radiation was applied acutely at the late morula/early blastocyst stage. The effects were quantified by the sister chromatid exchange (SCE) assay. All three agents induced SCEs when used singly. /sup 3/H-thymidine was effective in inducing SCEs only at 250 Bq/ml, whereas UV light was effective at both fluences. Although caffeine did not induce SCEs when it was added before exposure to bromodeoxyuridine (BrdUrd), which is used to visualize SCEs, it did induce SCEs when present during the entire culture period (/sup 3/H-thymidine experiments) or during incubation in BrdUrd (UV experiments). Caffeine markedly enhanced the SCE-inducing effect of UV light but did not influence the effect of /sup 3/H-thymidine.
Research Organization:
Univ. of California, San Francisco
OSTI ID:
5120376
Journal Information:
Teratog., Carcinog., Mutagen.; (United States), Journal Name: Teratog., Carcinog., Mutagen.; (United States) Vol. 2; ISSN TCMUD
Country of Publication:
United States
Language:
English

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Related Subjects

560121 -- Radiation Effects on Cells-- External Source-- (-1987)
560122* -- Radiation Effects on Cells-- Internal Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANALEPTICS
ANIMALS
AROMATICS
AZAARENES
AZINES
BIOLOGICAL EFFECTS
CAFFEINE
CENTRAL NERVOUS SYSTEM AGENTS
CHROMOSOMAL ABERRATIONS
DRUGS
ELECTROMAGNETIC RADIATION
EMBRYOS
GENETIC EFFECTS
HETEROCYCLIC COMPOUNDS
IN VITRO
LABELLED COMPOUNDS
MAMMALS
MICE
MUTATIONS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PURINES
PYRIMIDINES
RADIATIONS
RIBOSIDES
RODENTS
SISTER CHROMATID EXCHANGES
SYNERGISM
THYMIDINE
TOXICITY
TRITIUM COMPOUNDS
ULTRAVIOLET RADIATION
VERTEBRATES
XANTHINES