Elemental speciation by anion exchange and size exclusion chromatography with detection by inductively coupled plasma mass spectrometry with direct injection nebulization
- Ames Lab., IA (United States) Iowa State Univ., Ames, IA (United States)
A direct injection nebulizer (DIN) is used with packed microcolumns for anion chromatography (AC) and size exclusion chromatography (SEC). Two Se species (SeO[sub 3][sup 2[minus]] and SeO[sub 4][sup 2[minus]]) are separated by AC with detection limits of 15 pg of Se. The isotope ratio [sup 74]Se/[sup 78]Se can be measured with a relative standard deviation of 0.5% on 25 ng of each Se species during the actual chromatographic separation. Proteins in human serum are separated by SEC without sample pretreatment. The metals present in each molecular weight fraction are determined by ICP-MS with detection limits of 0.5-3 pg of metal. These absolute detection limits are 1-2 orders of magnitude better than those obtained with conventional nebulizers. 56 refs., 3 figs., 7 tabs.
- DOE Contract Number:
- W-7405-ENG-82
- OSTI ID:
- 5119411
- Journal Information:
- Analytical Chemistry (Washington); (United States), Vol. 65:21; ISSN 0003-2700
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES
METALLOPROTEINS
MICROANALYSIS
MOLECULAR WEIGHT
SEPARATION PROCESSES
SELENATES
ANIONS
BLOOD SERUM
DETECTION
EXPERIMENTAL DATA
GEL PERMEATION CHROMATOGRAPHY
INDUCTION
ION EXCHANGE CHROMATOGRAPHY
ISOTOPE RATIO
MASS SPECTROSCOPY
PLASMA HEATING
SELENIUM 74
SELENIUM 78
CHARGED PARTICLES
CHROMATOGRAPHY
DATA
EVEN-EVEN NUCLEI
HEATING
INFORMATION
INTERMEDIATE MASS NUCLEI
IONS
ISOTOPES
NUCLEI
NUMERICAL DATA
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PROTEINS
SELENIUM COMPOUNDS
SELENIUM ISOTOPES
SPECTROSCOPY
STABLE ISOTOPES
400100* - Analytical & Separations Chemistry
550200 - Biochemistry
400201 - Chemical & Physicochemical Properties