Elemental speciation by anion exchange and size exclusion chromatography with detection by inductively coupled plasma mass spectrometry with direct injection nebulization

Journal Article · Mon Nov 01 04:00:00 EST 1993 · Analytical Chemistry (Washington); (United States)

DOI:https://doi.org/10.1021/ac00069a006· OSTI ID:5119411

Shum, S C.K.; Houk, R S ^[1]

---

1. Ames Lab., IA (United States) Iowa State Univ., Ames, IA (United States)

A direct injection nebulizer (DIN) is used with packed microcolumns for anion chromatography (AC) and size exclusion chromatography (SEC). Two Se species (SeO[sub 3][sup 2[minus]] and SeO[sub 4][sup 2[minus]]) are separated by AC with detection limits of 15 pg of Se. The isotope ratio [sup 74]Se/[sup 78]Se can be measured with a relative standard deviation of 0.5% on 25 ng of each Se species during the actual chromatographic separation. Proteins in human serum are separated by SEC without sample pretreatment. The metals present in each molecular weight fraction are determined by ICP-MS with detection limits of 0.5-3 pg of metal. These absolute detection limits are 1-2 orders of magnitude better than those obtained with conventional nebulizers. 56 refs., 3 figs., 7 tabs.

• DOE Contract Number: W-7405-ENG-82
• OSTI ID: 5119411
• Journal Information: Analytical Chemistry (Washington); (United States), Journal Name: Analytical Chemistry (Washington); (United States) Vol. 65:21; ISSN 0003-2700; ISSN ANCHAM
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400100* -- Analytical & Separations Chemistry
400201 -- Chemical & Physicochemical Properties
550200 -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANIONS
BLOOD SERUM
CHARGED PARTICLES
CHROMATOGRAPHY
DATA
DETECTION
EVEN-EVEN NUCLEI
EXPERIMENTAL DATA
GEL PERMEATION CHROMATOGRAPHY
HEATING
INDUCTION
INFORMATION
INTERMEDIATE MASS NUCLEI
ION EXCHANGE CHROMATOGRAPHY
IONS
ISOTOPE RATIO
ISOTOPES
MASS SPECTROSCOPY
METALLOPROTEINS
MICROANALYSIS
MOLECULAR WEIGHT
NUCLEI
NUMERICAL DATA
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PLASMA HEATING
PROTEINS
SELENATES
SELENIUM 74
SELENIUM 78
SELENIUM COMPOUNDS
SELENIUM ISOTOPES
SEPARATION PROCESSES
SPECTROSCOPY
STABLE ISOTOPES