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Protein kinase C is involved in stimulation of arachidonic acid metabolism in Madin-Darby canine kidney (MDCK) cells

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:5118811
; ;

The authors used 12-O-tetradecanoyl-phorbol-13-acetate (TPA) to directly stimulate protein kinase C (PKC) in order to examine the role of PKC in transduction of biological signals that increase metabolism of arachidonic acid. Release of radioactive arachidonic acid and prostaglandins from TPA-stimulated MDCK cells is inhibited by either of two PKC inhibitors: 1-(5-isoquinolinesulfonyl)piperazine and 1-octadecyl-2-methoxy-glycero-3-phosphocholine (ALP). ALP is unable to inhibit cyclooxygenase when added into an in vitro assay for this enzyme. Furthermore, TPA induces de novo synthesis of cyclooxygenase in MDCK cells but ALP fails to prevent this effect of TPA. Thus, cyclooxygenase activity appears to be independent of PKC and TPA can still induce de novo synthesis of cyclooxygenase even in the presence of the PKC inhibitor ALP. Also, ALP has no effect on the release of arachidonic acid which occurs upon addition of the calcium ionophore A23187 to MDCK cells suggesting that there are multiple mechanisms to mobilize arachidonic acid. Their data indicate that activation of PKC by TPA leads to increased release of arachidonic acid through regulation of phospholipase(s) by PKC.

Research Organization:
Wake Forest Univ., Winston-Salem, NC
OSTI ID:
5118811
Report Number(s):
CONF-8606151-
Journal Information:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:6; ISSN FEPRA
Country of Publication:
United States
Language:
English

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Related Subjects

550200 -- Biochemistry
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ARACHIDONIC ACID
BIOLOGICAL EFFECTS
BODY
CARBOXYLESTERASES
CARBOXYLIC ACIDS
CARCINOGENS
CATS
ENZYME ACTIVITY
ENZYMES
ESTERASES
ESTERS
HYDROLASES
KIDNEYS
LIPASE
MAMMALS
METABOLISM
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
OXYGENASES
PHORBOL ESTERS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PROSTAGLANDINS
TRANSFERASES
VERTEBRATES