Mutagenic activities of heterocyclic amines in Chinese hamster lung cells in culture
A mutation assay system with Chinese hamster lung cells (CHL) using diphtheria toxin resistance as a selective marker has been established. The mutagenic activities of heterocyclic amines, originally isolated from pyrolyzates of amino acids and proteins, broiled fish and fried beef were assayed in cultured CHL cells in the absence and presence of a metabolic activation system, with diphtheria toxin resistance as a marker. All the heterocyclic amines tested except 3-amino-1,4-dimethyl-5H-pyrido (4,3-b)indole (Trp-P-1) required the presence of a metabolic activation system for mutagenicity on CHL cells. 3-Amino-1-methyl-5H-pyrido(4,3-b)indole (Trp-P-2) was the most mutagenic among the heterocyclic amines tested. Other compounds were also mutagenic in the following order of decreasing potency: Trp-P-1, 2-amino-3,4-dimethylimidazo(4,5-f)quinoline (MeIQ), 2-amino-3-methylimidazo(4,5-f)quinoline (IQ), 2-amino-9H-pyrido(2,3-b)indole (A..cap alpha..C), 2-amino-3,8-dimethylimidazo(4,5-f)quinoxaline (MeIQx), 2-amino-6-methyldipyrido(1,2-a:3',2'-d)imidazole (Glu-P-1) and 2-aminodipyrido(1,2--a:3',2'-d)imidazole (Glu-P-2).
- Research Organization:
- National Cancer Center Research Institute, Tokyo, Japan
- OSTI ID:
- 5118680
- Journal Information:
- Environ. Health Perspect.; (United States), Vol. 67
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ANIMAL CELLS
MUTATION FREQUENCY
HETEROCYCLIC COMPOUNDS
MUTAGEN SCREENING
AMINES
CELL CULTURES
DIPHTHERIA
HAMSTERS
INDOLES
LUNGS
MUTANTS
QUINOLINES
TOXINS
ANIMALS
ANTIGENS
AROMATICS
AZAARENES
AZINES
AZOLES
BACTERIAL DISEASES
BODY
DISEASES
INFECTIOUS DISEASES
MAMMALS
MATERIALS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIDINES
PYRROLES
RESPIRATORY SYSTEM
RODENTS
SCREENING
TOXIC MATERIALS
VERTEBRATES
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)