Effects of alcohol inhalation on the fatty acid composition of rat tissues
The objective of this study was to determine if polyunsaturated fatty acid levels in rat tissues are altered by alcohol exposure. Therefore, rats were exposed to ethanol vapor using an inhalation system designed in their laboratory and capable of producing rats with blood ethanol concentrations (BEC) of 50-400 mg%, as desired. Mean BEC were determined after 7 or 14 days of exposure and the lipids were extracted from brains, hearts, livers and aortas by the method of Bligh and Dyer. These total lipid extracts were transmethylated with BF/sub 3/ in methanol and analyzed using a fused silica capillary column. The largest compositional changes were observed in the liver after 14 days of exposure; 18:0, 18:2w6 and 20:4w6 decreased by 7, 18, and 20%, respectively, whereas 16:1w7, 18:1w9, 18:3w3 and 22:6w3 increased by 42, 19, 9, and 11%, respectively. A similar, although quantitatively smaller, effect was observed after only 7 days of alcohol inhalation. Little change was observed in the total lipid extract fatty acyl distribution in the brain or heart, however, the aorta showed losses in the polyunsaturates 20:4w6 and 22:6w3. More detailed data will be presented for the fatty acid distributional changes in individual phospholipid classes. It appears that the direction and magnitude of compositional changes depend upon both the duration of exposure and the blood concentration of alcohol.
- Research Organization:
- NIAAA, Bethesda, MD
- OSTI ID:
- 5114553
- Report Number(s):
- CONF-8606151-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:6; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986; Other Information: C52.30*
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CARBOXYLIC ACIDS
CHEMICAL COMPOSITION
ETHANOL
BIOLOGICAL EFFECTS
AORTA
BRAIN
HEART
INHALATION
LIVER
RATS
ALCOHOLS
ANIMALS
ARTERIES
BLOOD VESSELS
BODY
CARDIOVASCULAR SYSTEM
CENTRAL NERVOUS SYSTEM
DIGESTIVE SYSTEM
GLANDS
HYDROXY COMPOUNDS
INTAKE
MAMMALS
NERVOUS SYSTEM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
RODENTS
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)