DNA repair after ultraviolet irradiation of ICR 2A frog cells: pyrimidine dimers are long acting blocks to nascent DNA synthesis
Journal Article
·
· Biophys. J.; (United States)
The ability of ICR 2A frog cells to repair DNA damage induced by ultraviolet irradiation was examined. These cells are capable of photoreactivation but are nearly totally deficient in excision repair. They have the ability to convert the small molecular weight DNA made after irradiation into large molecules but do not show an enhancement in this process when the UV dose is delivered in two separate exposures separated by a 3- or 24-h incubation. Total DNA synthesis is depressed and low molecular weight DNA continues to be synthesized during pulse-labeling as long as 48 h after irradiation. The effects of pyrimidine dimer removal through exposure of UV irradiated cells to photoreactivating light indicate that dimers act as the critical lesions blocking DNA synthesis.
- Research Organization:
- Brookhaven National Lab., Upton, NY
- OSTI ID:
- 5099839
- Journal Information:
- Biophys. J.; (United States), Journal Name: Biophys. J.; (United States) Vol. 31:2; ISSN BIOJA
- Country of Publication:
- United States
- Language:
- English
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Inhibition of semiconservative DNA synthesis in ICR 2A frog cells exposed to monochromatic uv wavelengths (252-313 nm) and photoreactivating light
Journal Article
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Thu Aug 01 00:00:00 EDT 1985
· Radiat. Res.; (United States)
·
OSTI ID:5321714
Inhibition of semiconservative DNA synthesis in ICR 2A frog cells by pyrimidine dimers and nondimer photoproducts induced by ultraviolet radiation
Journal Article
·
Wed Oct 31 23:00:00 EST 1984
· Radiat. Res.; (United States)
·
OSTI ID:6054968
Inhibition of semiconservative DNA synthesis in ICR 2A frog cells exposed to monochromatic uv wavelengths (252-313 nm) and photoreactivating light
Journal Article
·
Tue Jun 01 00:00:00 EDT 1982
· Radiat. Res.; (United States)
·
OSTI ID:7086102
Related Subjects
550200 -- Biochemistry
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMPHIBIANS
ANIMAL CELLS
ANIMALS
AQUATIC ORGANISMS
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOLOGY
BIOSYNTHESIS
CELL CULTURES
DIMERS
DNA
DOSES
ELECTROMAGNETIC RADIATION
FROGS
GENETICALLY SIGNIFICANT DOSE
GENETICS
HETEROCYCLIC COMPOUNDS
MOLECULAR WEIGHT
MORPHOLOGICAL CHANGES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOTOREACTIVATION
PYRIMIDINES
RADIATION DOSES
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY
RECOVERY
REPAIR
STRAND BREAKS
SYNTHESIS
ULTRASTRUCTURAL CHANGES
ULTRAVIOLET RADIATION
VERTEBRATES
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMPHIBIANS
ANIMAL CELLS
ANIMALS
AQUATIC ORGANISMS
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOLOGY
BIOSYNTHESIS
CELL CULTURES
DIMERS
DNA
DOSES
ELECTROMAGNETIC RADIATION
FROGS
GENETICALLY SIGNIFICANT DOSE
GENETICS
HETEROCYCLIC COMPOUNDS
MOLECULAR WEIGHT
MORPHOLOGICAL CHANGES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOTOREACTIVATION
PYRIMIDINES
RADIATION DOSES
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY
RECOVERY
REPAIR
STRAND BREAKS
SYNTHESIS
ULTRASTRUCTURAL CHANGES
ULTRAVIOLET RADIATION
VERTEBRATES