Increase in immunogenicity with concomitant loss of tumorigenicity of respiratory tract carcinomas during in vitro culture

Journal Article · Sun Jul 01 04:00:00 EDT 1979 · Cancer Res.; (United States)

OSTI ID:5099046

Jamasbi, R J ^[1]; Nettesheim, P

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1. Iran School of Medicine amd Medical Sciences, Tehran

Cell lines were established in vitro from respiratory tract carcinomas induced in rats by carcinogenic, polycyclic hydrocarbons. Propagation of the carcinoma lines in vitro lead to a progressive decrease in tumorigenicity. Tumor transplantation studies in x-irradiated, immunosuppressed recipients and in immunologically reconstituted recipients suggested that the cells are rejected because of their immunogenicity, since a high incidence of tumors was observed in x-irradiated recipients but not in normal or x-irradiated, reconstituted recipients. When immunologically competent rats were immunized with cells from an in vitro tumor line, strong tumor transplantation resistance resulted. Similar immunization with the corresponding in vivo tumor line caused very little if any protection, and immunization with a non-cross-reacting sarcoma line grown in vitro did not produce immunological protection against carcinoma cell lines. A single in vivo passage of the in vitro-adapted tumor line in immunosuppressed recipients fully restored tumorigenicity. The increase in immunogenicity of carcinomas cultured in vitro appears to involve preexisting antigens indigenous to the carcinomas rather than new antigens acquired during tissue culture, such as antigens related to retroviruses, mycoplasmas, or heterologous serum.

• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 5099046
• Journal Information: Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 39; ISSN CNREA
• Country of Publication: United States
• Language: English

Related Subjects

550300 -- Cytology
550603* -- Medicine-- External Radiation in Therapy-- (1980-)
560305 -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIGENS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CARCINOMAS
CELL CULTURES
DISEASES
ELECTROMAGNETIC RADIATION
EXTERNAL IRRADIATION
GRAFT-HOST REACTION
IMMUNE REACTIONS
IMMUNOSUPPRESSION
IN VITRO
IONIZING RADIATIONS
IRRADIATION
MAMMALS
NEOPLASMS
RADIATION EFFECTS
RADIATIONS
RATS
RESPIRATORY TRACT CELLS
RESPONSE MODIFYING FACTORS
RODENTS
TUMOR CELLS
VERTEBRATES
WHOLE-BODY IRRADIATION
X RADIATION