Effects of light, oxygen, and substrates on steady-state levels of mRNA coding for ribulose-1,5-biosphosphate carboxylase and light-harvesting and reaction center polypeptides in Rhodopseudomonas sphaeroides

Journal Article · Sat Jun 01 04:00:00 EDT 1985 · J. Bacteriol.; (United States)

OSTI ID:5098154

Zhu, Y S; Kaplan, S

The mRNA levels specific for ribulose-1,5-bisphosphate carboxylase, light-harvesting 1 polypeptides ..cap alpha.. and ..beta.., and reaction center polypeptides L and M were assayed by use of a series of DNA probes specific for each cognate mRNA. Both the steady-state amounts and sizes of the specific mRNAs were measured as a function of the light intensity incident to the culture, the presence or absence of oxygen, and the type of substrate present in the growth medium. Northern hybridization revealed at least two and possibly three transcripts for ribulose-1,5-bisphosphate carboxylase. The cellular level of mRNA specific for ribulose-1,5-bisphosphate carboxylase increased in consort with enzyme activity as a function of both light intensity and reducing state of the substrate. Neither mRNA nor enzyme activity was detectable in aerobically grown cells. For the light-harvesting 1 and reaction center polypeptides there exist two transcripts, the larger of which appears to be a polycistronic mRNA possessing information for all four polypeptides and a smaller transcript specific for only the ..cap alpha.. and ..beta.. polypeptides of the light-harvesting 1 complex. The regulation of each of these mRNAs was affected by light and oxygen, but was not significantly affected by the oxidation-reduction state of the substrate.

• Research Organization: Univ. of Illinois, Urbana-Champaign
• OSTI ID: 5098154
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 162:3; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BIOCHEMISTRY
BIOLOGICAL EFFECTS
CARBON-CARBON LYASES
CARBOXY-LYASES
CARBOXYLASE
CHEMISTRY
DNA
ELECTROMAGNETIC RADIATION
ELEMENTS
ENZYME ACTIVITY
ENZYMES
LYASES
MESSENGER-RNA
MICROORGANISMS
NONMETALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
OXYGEN
PEPTIDES
PHOTOSYNTHETIC REACTION CENTERS
POLYPEPTIDES
PROTEINS
RADIATIONS
RECOMBINANT DNA
RHODOPSEUDOMONAS
RNA
TRANSCRIPTION
VISIBLE RADIATION