Radioprotector WR1065 reduces radiation-induced mutations at the hypoxanthine-guanine phosphoribosyl transferase locus in V79 cells

Journal Article · Sat Jun 01 04:00:00 EDT 1985 · Carcinogenesis (N.Y.); (United States)

DOI:https://doi.org/10.1093/carcin/6.6.929· OSTI ID:5094909

Grdina, D J; Hill, C K; Peraino, C ^[1]; Biserka, N ^[2]; Wells, R L ^[3]

---

1. Argonne National Lab., IL (USA)
2. Central Inst. for Tumors and Allied Diseases, Zagreb (Yugoslavia)
3. Colorado State Univ., Fort Collins (USA). Dept. of Radiology and Radiation Biology

N-(2-mercaptoethyl)-1,3-diaminopropane (WR1065) protects against radiation-induced cell killing and mutagenesis at the hypoxanthine-guanine phosphoribosyl transferase (HGPRT) locus in V79 Chinese hamster lung fibroblast cells. WR1065 (4 mm) was found to be effective in protecting against radiation-induced cell lethality only if present during irradiation. No protective effect was observed if the protector was added within 5 min after irradiation or 3 h later. The effect of WR1065 on radiation-induced mutation, expressed as resistance to the cytotoxic purine analogue 6-thioguanine (HGPRT), was also investigated. This agent was effective in reducing radiation-induced mutations regardless of when it was administered. Following 10 Gy of /sup 60/Co ..gamma..-rays, the mutation frequencies observed per 10/sup 6/ survivors were 77 +- 8, 27 +- 6, 42 +- 7, and 42 +- 7 for radiation only, and WR1065 present during, immediately after, or 3 h after irradiation. These data suggest that although a segment of radiation-induced damage leading to reproductive death cannot be modulated through the postirradiation action of WR1065, processes leading to the fixation of gross genetic damage and mutation induction in surviving cells can be effectively altered and interfered with leading to a marked reduction in mutation frequency.

• OSTI ID: 5094909
• Journal Information: Carcinogenesis (N.Y.); (United States), Journal Name: Carcinogenesis (N.Y.); (United States) Vol. 6:6; ISSN CRNGD
• Country of Publication: United States
• Language: English

Related Subjects

560114* -- Radiation Effects on Biochemicals-- In Animals-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL CULTURES
CELL KILLING
CONNECTIVE TISSUE CELLS
DRUGS
ELECTROMAGNETIC RADIATION
ENZYMES
FIBROBLASTS
GAMMA RADIATION
GENE MUTATIONS
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
GLYCOSYL TRANSFERASES
HAMSTERS
HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE
IONIZING RADIATIONS
MAMMALS
MUTATION FREQUENCY
MUTATIONS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PENTOSYL TRANSFERASES
RADIATION EFFECTS
RADIATIONS
RADIOPROTECTIVE SUBSTANCES
RODENTS
SOMATIC CELLS
SURVIVAL CURVES
THIOLS
TIME DEPENDENCE
TRANSFERASES
VERTEBRATES