Effect of sepsis on VLDL kinetics: responses in basal state and during glucose infusion
The effect of gram-negative sepsis on the kinetics and oxidation of very low-density lipoprotein (VLDL) fatty acids was assessed in conscious dogs in the normal state and 24 h after infusion of live Escherichia coli. VLDL, labeled with (2-/sup 3/H)glycerol and (1-/sup 14/C)palmitic acid, was used to trace VLDL kinetics and oxidation, and (1-/sup 13/C)palmitic acid bound to albumin was infused simultaneously to quantify kinetics and oxidation of free fatty acid (FFA) in plasma. Sepsis caused a fivefold increase in the rate of VLDL production (RaVLDL). In the control dogs, the direct oxidation of VLDL-fatty acids was not an important contributor to their overall energy metabolism, but in dogs with sepsis, 17% of the total rate of CO2 production could be accounted for by VLDL-fatty acid oxidation. When glucose was infused into dogs with insulin and glucagon levels clamped at basal levels (by means of infusion of somatostatin and replacement of the hormones), RaVLDL increased significantly in the control dogs, but it did not increase further in dogs with sepsis. The authors conclude that the increase in triglyceride concentration in fasting dogs with gram-negative sepsis is the result of an increase in VLDL production and that the fatty acids in VLDL can serve as an important source of energy in sepsis.
- OSTI ID:
- 5079876
- Journal Information:
- Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States); ISSN AJPHA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
ALCOHOLS
ALDEHYDES
ANIMALS
BACTERIA
BIOCHEMICAL REACTION KINETICS
CARBOHYDRATES
CARBON 13
CARBON 14 COMPOUNDS
CARBON ISOTOPES
CARBOXYLIC ACIDS
DOGS
ESCHERICHIA COLI
ESTERS
EVEN-ODD NUCLEI
GLUCOSE
GLYCEROL
HEXADECANOIC ACID
HEXOSES
HYDROXY COMPOUNDS
INFECTIVITY
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LIGHT NUCLEI
LIPIDS
LIPOPROTEINS
MAMMALS
METABOLISM
MICROORGANISMS
MONOCARBOXYLIC ACIDS
MONOSACCHARIDES
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
REACTION KINETICS
SACCHARIDES
STABLE ISOTOPES
TRACER TECHNIQUES
TRIGLYCERIDES
TRITIUM COMPOUNDS
VERTEBRATES