Evaluation of an exposure system using cells grown on collagen gels for detecting highly volatile mutagens in the CHO/HGPRT mutation assay
Chinese hamster ovary (CHO) cells were grown on hydrated collagen gels, the overlaying medium removed leaving the cells at an air/collagen interface, and the cells exposed to a dynamic flow of ethylene oxide. Increases in CHO cell mutant frequency and decreases in cell viability were observed. To establish if the exposure system could be simplified, cells were exposed in sealed bottles (static system) to ethylene oxide. No substantial changes in cytotoxicity, mutant frequency, or effective concentration were noted when comparing static versus dynamic exposure systems. The general usefulness of the exposure system using cells grown on collagen gels was evaluated in a static system using propylene oxide and 1,2-dichloroethane, both of which were found to be mutagenic and cytotoxic. Comparatively, the exposure of cells by the collagen gel method was as effective in detecting genotoxic damage as were conventional methods (cells covered with medium) using cells grown on glass substrates. The exposure of CHO cells on collagen gels to highly volatile mutagens was simple and inexpensive, and may be generally useful in the detection of gaseous or volatile mutagens.
- Research Organization:
- Inhalation Toxicology Research Inst., Albuquerque, NM
- DOE Contract Number:
- AC04-76EV01013
- OSTI ID:
- 5073741
- Journal Information:
- Environ. Manage.; (United States), Vol. 5:6
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
CHO CELLS
MUTATION FREQUENCY
EPOXIDES
MUTAGEN SCREENING
CHLORINATED ALIPHATIC HYDROCARBONS
COLLAGEN
HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE
ANIMAL CELLS
ENZYMES
GLYCOSYL TRANSFERASES
HALOGENATED ALIPHATIC HYDROCARBONS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PENTOSYL TRANSFERASES
PROTEINS
SCLEROPROTEINS
SCREENING
TRANSFERASES
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)
550400 - Genetics