Mutational specificity in mammalian cells: Progress report for period February 1, 1987-January 31, 1988: (Final report)

Technical Report · Tue Mar 29 04:00:00 EST 1988

OSTI ID:5069300

Strauss, B S

This paper discusses the termination of in vitro DNA synthesis where the nature of the lesion as well as the polymerase determine the specificity of base inserted opposite the lesion. We confirmed the A rule at abasic sites produced by the removal of cytosine from the DNA. Treatment of SNA with dimethylsulfate or N-methyl-N'-nitro-N-nitrosoguanidine leads to transcription termination at site of the immediately preceding A indicating that methylated G's are not blocks and supporting that 3-methyladenine is a lethal lesion where DNA synthesis terminates. By using both uv- and methylation lesions, the rules for termination worked out using single stranded DNA molecules as templates were shown to hold for double stranded substrates. A system for measurement of mutation using a M13 vector containing a lac complementing insert and a uracil containing (/plus/) strand annealed to a normal (/minus/) strand but without the insert so that the lac complementing region forms a single stranded gap was developed. 3 figs., 4 tabs.

• Research Organization: Chicago Univ., IL (USA)
• DOE Contract Number: AC02-76EV02040
• OSTI ID: 5069300
• Report Number(s): DOE/EV/02040-; ON: DE88008478
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETYLAMINOFLUORENES
AMINES
DNA BASE TRANSITIONS
DNA SEQUENCING
DOCUMENT TYPES
MUTAGENESIS
ORGANIC COMPOUNDS
POLYCYCLIC AROMATIC AMINES
PROGRESS REPORT
STRUCTURAL CHEMICAL ANALYSIS