Illegitimate recombination induced by DNA double-strand breaks in a mammalian chromosome
Journal Article
·
· Molecular and Cellular Biology
- Univ. of California, San Francisco, CA (United States)
Restriction endonucleases were electroporated into CHO cells to produce blunt-end DNA double-strand breaks in the adenine phosphoribosyltransferase (APRT) locus. Viable colonies containing APRT mutations were expanded and the DNA was sequenced. The results indicate that blunt-end DNA double-strand breaks can induce illegitimate (nonhomologous) recombination in mammalian chromosomes and that they play an important role in mutagenesis. 53 refs., 7 figs., 3 tabs.
- Research Organization:
- OAK (Oakland Operations Office, Oakland, CA)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC03-76SF01012
- OSTI ID:
- 50638
- Journal Information:
- Molecular and Cellular Biology, Journal Name: Molecular and Cellular Biology Journal Issue: 9 Vol. 14; ISSN 0270-7306; ISSN MCEBD4
- Country of Publication:
- United States
- Language:
- English
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