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Ultra violet-endonuclease from calf thymus with specificity toward pyrimidine dimers in DNA

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
 [1]; ;
  1. Tel-Aviv Univ., Israel
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We describe the partial purification of an endonuclease from calf thymus that nicks phage PM2 DNA irradiated with uv doses producing only a few pyrimidine dimers per molecule. It has much less activity on DNA that has been subjected to enzymatic photoreactivation after uv irradiation. The calf thymus endonuclease is different from other mammalian uv-endonucleases so far described in that it seems to be dimer specific. The enzyme is stimulated by Mg/sup 2 +/ and is inactive in the presence of EDTA. It binds to uv-irradiated DNA-Sepharose from which it is released by low concentrations of KCl. Gel filtration data indicate that the endonuclease may belong to a high molecular weight protein or protein complex. The enzyme is very labile and freezing increases it lability.
OSTI ID:
5061968
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 76:8; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

550200 -- Biochemistry
560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
ANIMALS
AZINES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
CARBOXYLIC ACIDS
CATTLE
CHARGED PARTICLES
CHELATING AGENTS
DIMERS
DNA
DOMESTIC ANIMALS
EDTA
ELECTROMAGNETIC RADIATION
ENZYME ACTIVITY
ENZYMES
HETEROCYCLIC COMPOUNDS
IONS
KINETICS
LYMPHATIC SYSTEM
MAGNESIUM IONS
MAMMALS
MOLECULAR WEIGHT
NUCLEASES
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PHOSPHOTRANSFERASES
PURIFICATION
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
REACTION KINETICS
RECOVERY
REPAIR
RUMINANTS
SPECIFICITY
THYMUS
TRANSFERASES
ULTRAVIOLET RADIATION
VERTEBRATES