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Proteins of human milk. I. Identification of major components

Journal Article · · Clin. Chem. (Winston-Salem, N.C.); (United States)
OSTI ID:5054150
; ;
Traditionally, human milk proteins are identified largely by reference to bovine milk. Hence, to identify the major proteins in human milk, we subjected human and bovine milk, in parallel, to high-resolution two-dimensional electrophoresis. Isoelectric precipitation at pH 4.6 was our criterion for distinguishing whey proteins from those of the casein complex. The ..cap alpha..- and..beta..-caseins were identified on the basis of relative abundance, relative molecular mass, and relative isoelectric points. No protein disappeared from ISO-DALT patterns of human milk after rennin treatment, and no new protein comparable to bovine para K-casein appeared in the BASO-DALT patterns; this suggests that K-casein is absent from human milk. The proteins identified in human milk patterns include the ..cap alpha.. and ..beta.. casein families, lactalbumin, albumin, transferrin, IgA, and lactoferrin. Numerous additional proteins seen in patterns for human milk remain to be identified.
Research Organization:
Argonne National Lab., IL
DOE Contract Number:
W-31109-ENG-38
OSTI ID:
5054150
Journal Information:
Clin. Chem. (Winston-Salem, N.C.); (United States), Journal Name: Clin. Chem. (Winston-Salem, N.C.); (United States) Vol. 28:4; ISSN CLCHA
Country of Publication:
United States
Language:
English

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Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
ANIMALS
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BODY FLUIDS
CASEIN
CATTLE
CHEMISTRY
COMPARATIVE EVALUATIONS
COWS
DOMESTIC ANIMALS
ELECTROPHORESIS
ENZYMES
FOOD
GLOBULINS
GLOBULINS-BETA
HYDROLASES
IMMUNOGLOBULINS
INDUSTRIAL WASTES
MAMMALS
MAN
MATERIALS
METALLOPROTEINS
MILK
NONSPECIFIC PEPTIDASES
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PEPTIDE HYDROLASES
PRIMATES
PROTEINS
RENIN
RUMINANTS
TRANSFERRIN
VERTEBRATES
WASTES
WHEY