Specific estrogen-induced cell proliferation of cultured Syrian hamster renal proximal tubular cells in serum-free chemically defined media

Journal Article · Wed Mar 01 04:00:00 EST 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.6.2107· OSTI ID:5052297

Oberley, T D; Lauchner, L J; Pugh, T D; Gonzalez, A; Goldfarb, S ^[1]; Li, S A; Li, J J ^[2]

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1. Univ. of Wisconsin Medical School, Madison (USA)
2. Univ. of Minnesota Medical School, Minneapolis (USA)

It has long been recognized that the renal proximal tubular epithelium of the hamster is a bona fide estrogen target tissue. The effect of estrogens on the growth of proximal tubule cell explants and dissociated single cells derived from these explant outgrowths has been studied in culture. Renal tubular cells were grown on a PF-HR-9 basement membrane under serum-free chemically defined culture conditions. At 7-14 days in culture, cell number was enhanced 3-fold in the presence of either 17{beta}-estradiol or diethylstilbestrol. A similar 3-fold increase in cell number was also seen at 1 nM 17{beta}-estradiol in subcultured dissociated single tubular cells derived from hamster renal tubular explant outgrowths at 21 days in culture. Concomitant exposure of tamoxifen at 3-fold molar excess in culture completely abolished the increase in cell number seen with 17{beta}-estradiol. The proliferation effect of estrogens on proximal tubular cell growth appears to be species specific since 17{beta}-estradiol did not alter the growth of either rat or guinea pig proximal tubules in culture. In addition, at 7-10 days in culture in the presence of 17{beta}-estradiol, ({sup 3}H)thymidine labeling of hamster tubular cells was enhanced 3-fold. These results clearly indicate that estrogens can directly induce primary epithelial cell proliferation at physiologic concentrations and provide strong additional evidence for an important hormonal role in the neoplastic transformation of the hamster kidney.

• OSTI ID: 5052297
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 86:6; ISSN PNASA; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AZINES
BIOLOGICAL EFFECTS
BODY
CARCINOGENESIS
CELL CULTURES
CELL PROLIFERATION
CELL TRANSFORMATIONS
CYTOLOGICAL TECHNIQUES
ESTROGENS
GUINEA PIGS
HAMSTERS
HETEROCYCLIC COMPOUNDS
HORMONES
HYDROGEN COMPOUNDS
KIDNEYS
MAMMALS
NUCLEOSIDES
NUCLEOTIDES
ONCOGENIC TRANSFORMATIONS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PATHOGENESIS
PYRIMIDINES
RATS
RIBOSIDES
RODENTS
STEROID HORMONES
THYMIDINE
TRITIUM COMPOUNDS
VERTEBRATES