Polymethylmethacrylate-induced release of bone-resorbing factors

Journal Article · Fri Dec 01 04:00:00 EST 1989 · Journal of Bone and Joint Surgery, American Volume; (USA)

OSTI ID:5047185

Herman, J H; Sowder, W G; Anderson, D; Appel, A M; Hopson, C N ^[1]

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1. Univ. of Cincinnati College of Medicine, OH (USA)

A pseudomembranous structure that has the histological characteristics of a foreign-body-like reaction invariably develops at the bone-cement interface in the proximity of resorption of bone around aseptically loosened cemented prostheses. This study was an attempt to implicate polymethylmethacrylate in this resorptive process. Unfractionated peripheral-blood mononuclear cells (consisting of lymphocytes and monocytes) and surface-adherent cells (monocyte-enriched) were prepared from control subjects who did and did not have clinical evidence of osteoarthrosis and from patients who had osteoarthrosis and were having a revision for failure of a cemented hip or knee implant. Cells were cultured for varying periods in the presence and absence of nonpolymerized methacrylate (one to two-micrometer spherules), pulverized polymerized material, or culture chambers that were pre-coated with polymerized cement. Conditioned media that were derived from both methacrylate-stimulated cell populations were shown to contain specific bone-resorbing mediators (interleukin-1, tumor necrosis factor, or prostaglandin E2) and to directly affect bone resorption in 45Ca-labeled murine limb-bone assays.

• OSTI ID: 5047185
• Journal Information: Journal of Bone and Joint Surgery, American Volume; (USA), Journal Name: Journal of Bone and Joint Surgery, American Volume; (USA) Vol. 71:10; ISSN JBJSA; ISSN 0021-9355
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH ISOTOPES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
BONE JOINTS
CALCIUM 45
CALCIUM ISOTOPES
CARBOXYLIC ACID SALTS
CELL CULTURES
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DISEASES
EVEN-ODD NUCLEI
GROWTH FACTORS
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
LYMPHOCYTES
LYMPHOKINES
MAMMALS
MATERIALS
MEDICAL SUPPLIES
METHACRYLATES
MICE
MITOGENS
NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROSTAGLANDINS
PROSTHESES
PROTEINS
RADIOASSAY
RADIOISOTOPES
RODENTS
SKELETAL DISEASES
SKELETON
SOMATIC CELLS
TRACER TECHNIQUES
VERTEBRATES