Uptake of low molecular weight materials in an in situ preparation of the rabbit thoracic aorta
1. An in situ preparation of the rabbit thoracic aorta is described in which the integrity of the intimal endothelium is shown to be preserved. An extensive system of vasa vasorum and lymphatic vessels distributed throughout the adventitia, but not penetrating the media, are demonstrated using resin casts and lead chromate precipitation techniques and are shown to be patent in this preparation. 2. The role of the vasa vasorum in the medial uptake of low molecular weight materials is investigated as a function of luminal hydrostatic pressure and perfusion time. Comparison of uptake in vessels with and without patent vasa vasorum indicates that at physiological pressures medial uptake of 125I ions is dominated by entry from the adventitial surface. Analysis of the transmural distribution of 125I demonstrates that iodide is not retarded at the endothelial surface but a barrier exists within the intima which is tentatively identified as the internal elastic lamina. Qualitative studies show that Patent Blue V whose coloured anion has a molecular weight of 500 is able, at longer perfusion times, to penetrate this barrier. There is no circumferential variation in intimal uptake, but in contrast, adventitial uptake is rather patchy and generally more intense on the dorsal surfaces of the aorta. 3. Iodide transport across the intimal surface is argued to be predominantly diffusive in short duration experiments with an effective diffusion coefficient of (1.6 +/- 0.9) X 10(-6) cm2 min-1.
- Research Organization:
- Physiological Flow Studies Unit, Imperial College, London
- OSTI ID:
- 5047169
- Journal Information:
- Artery (Fulton, Mich.); (United States), Vol. 9:6
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ANIONS
UPTAKE
AORTA
PHYSIOLOGY
CELL MEMBRANES
PERMEABILITY
IODINE 125
DIFFUSION
MOLECULAR WEIGHT
RABBITS
TRACER TECHNIQUES
ANIMALS
ARTERIES
BETA DECAY RADIOISOTOPES
BLOOD VESSELS
BODY
CARDIOVASCULAR SYSTEM
CELL CONSTITUENTS
CHARGED PARTICLES
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
IONS
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MEMBRANES
NUCLEI
ODD-EVEN NUCLEI
ORGANS
RADIOISOTOPES
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques