Uptake of low molecular weight materials in an in situ preparation of the rabbit thoracic aorta

Journal Article · Thu Jan 01 04:00:00 EST 1981 · Artery (Fulton, Mich.); (United States)

OSTI ID:5047169

Davis, J; Winlove, C P

1. An in situ preparation of the rabbit thoracic aorta is described in which the integrity of the intimal endothelium is shown to be preserved. An extensive system of vasa vasorum and lymphatic vessels distributed throughout the adventitia, but not penetrating the media, are demonstrated using resin casts and lead chromate precipitation techniques and are shown to be patent in this preparation. 2. The role of the vasa vasorum in the medial uptake of low molecular weight materials is investigated as a function of luminal hydrostatic pressure and perfusion time. Comparison of uptake in vessels with and without patent vasa vasorum indicates that at physiological pressures medial uptake of 125I ions is dominated by entry from the adventitial surface. Analysis of the transmural distribution of 125I demonstrates that iodide is not retarded at the endothelial surface but a barrier exists within the intima which is tentatively identified as the internal elastic lamina. Qualitative studies show that Patent Blue V whose coloured anion has a molecular weight of 500 is able, at longer perfusion times, to penetrate this barrier. There is no circumferential variation in intimal uptake, but in contrast, adventitial uptake is rather patchy and generally more intense on the dorsal surfaces of the aorta. 3. Iodide transport across the intimal surface is argued to be predominantly diffusive in short duration experiments with an effective diffusion coefficient of (1.6 +/- 0.9) X 10(-6) cm2 min-1.

• Research Organization: Physiological Flow Studies Unit, Imperial College, London
• OSTI ID: 5047169
• Journal Information: Artery (Fulton, Mich.); (United States), Journal Name: Artery (Fulton, Mich.); (United States) Vol. 9:6; ISSN ARTED
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANIONS
AORTA
ARTERIES
BETA DECAY RADIOISOTOPES
BLOOD VESSELS
BODY
CARDIOVASCULAR SYSTEM
CELL CONSTITUENTS
CELL MEMBRANES
CHARGED PARTICLES
DAYS LIVING RADIOISOTOPES
DIFFUSION
ELECTRON CAPTURE RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
IONS
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MEMBRANES
MOLECULAR WEIGHT
NUCLEI
ODD-EVEN NUCLEI
ORGANS
PERMEABILITY
PHYSIOLOGY
RABBITS
RADIOISOTOPES
TRACER TECHNIQUES
UPTAKE
VERTEBRATES