Permeabilization of ultraviolet-irradiated chinese hamster cells with polyethylene glycol and introduction of ultraviolet endonuclease from Micrococcus luteus
Journal Article
·
· Mol. Cell. Biol.; (United States)
Chinese hamster V-79 cells were made permeable by treatment with polyethylene glycol and then incubated with a Micrococcus luteus extract containing ultraviolet-specific endonuclease activity. This treatment introduced nicks in irradiated, but not in unirradiated, deoxyribonucleic acid. The nicks remained open for at least 3 h; there was no loss of endonuclease-sensitive sites, and no excision of dimers as measured by chromatography was detected. In addition, there was no increase in ultraviolet resistance in treated cells. This suggests that the absence of a significant amount of excision repair in rodent cells is due to the lack of both incision and excision capacity.
- Research Organization:
- Brookhaven National Lab. (BNL), Upton, NY (United States)
- OSTI ID:
- 5046684
- Journal Information:
- Mol. Cell. Biol.; (United States), Vol. 1:3
- Country of Publication:
- United States
- Language:
- English
Similar Records
DNA repair in V-79 cells treated with combinations of ultraviolet radiation and N-acetoxy-2-acetylaminofluorene
Micrococcus luteus correndonucleases. II. Mechanism of action of two endonucleases specific for DNA containing pyrimidine dimers. [Micrococcus lutea; 15 J/m/sup 3/]
Modulation of the DNA scanning activity of the Micrococcus luteus UV endonuclease
Journal Article
·
Thu Sep 01 00:00:00 EDT 1977
· Cancer Res.; (United States)
·
OSTI ID:5046684
Micrococcus luteus correndonucleases. II. Mechanism of action of two endonucleases specific for DNA containing pyrimidine dimers. [Micrococcus lutea; 15 J/m/sup 3/]
Journal Article
·
Sun Sep 25 00:00:00 EDT 1977
· J. Biol. Chem.; (United States)
·
OSTI ID:5046684
Modulation of the DNA scanning activity of the Micrococcus luteus UV endonuclease
Journal Article
·
Sun Oct 15 00:00:00 EDT 1989
· Journal of Biological Chemistry; (USA)
·
OSTI ID:5046684
Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
DNA
BIOLOGICAL RADIATION EFFECTS
FIBROBLASTS
PERMEABILITY
NUCLEASES
RADIOSENSITIVITY EFFECTS
BIOLOGICAL REPAIR
CARBON 14 COMPOUNDS
DIMERS
EXTREME ULTRAVIOLET RADIATION
HAMSTERS
MICROCOCCUS LUTEUS
POLYETHYLENE GLYCOLS
RADIOINDUCTION
TRITIUM COMPOUNDS
ALCOHOLS
ANIMAL CELLS
ANIMALS
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
ENZYMES
ESTERASES
GLYCOLS
HYDROLASES
HYDROXY COMPOUNDS
LABELLED COMPOUNDS
MAMMALS
MICROCOCCUS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC POLYMERS
PHOSPHODIESTERASES
POLYMERS
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
RODENTS
SOMATIC CELLS
ULTRAVIOLET RADIATION
VERTEBRATES
560121* - Radiation Effects on Cells- External Source- (-1987)
DNA
BIOLOGICAL RADIATION EFFECTS
FIBROBLASTS
PERMEABILITY
NUCLEASES
RADIOSENSITIVITY EFFECTS
BIOLOGICAL REPAIR
CARBON 14 COMPOUNDS
DIMERS
EXTREME ULTRAVIOLET RADIATION
HAMSTERS
MICROCOCCUS LUTEUS
POLYETHYLENE GLYCOLS
RADIOINDUCTION
TRITIUM COMPOUNDS
ALCOHOLS
ANIMAL CELLS
ANIMALS
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
ENZYMES
ESTERASES
GLYCOLS
HYDROLASES
HYDROXY COMPOUNDS
LABELLED COMPOUNDS
MAMMALS
MICROCOCCUS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC POLYMERS
PHOSPHODIESTERASES
POLYMERS
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
RODENTS
SOMATIC CELLS
ULTRAVIOLET RADIATION
VERTEBRATES
560121* - Radiation Effects on Cells- External Source- (-1987)