Optimization and characterization of an in vitro bovine mammary cell culture system to study regulation of milk protein synthesis and mammary differentiation
Thesis/Dissertation
·
OSTI ID:5045196
A long term bovine mammary cell culture system that maintains normal mammary cell function was established and optimized to study milk protein synthesis and secretion and mammary differentiation. This culture system used bovine mammary acini isolated from developing or lactating mammary gland by enzymatic dissociation, and cryopreserved until thawed and plated for growth in vitro for these studies. Cells in M199 with lactogenic hormones {plus minus} fetal calf serum (FCS) were cultured on plastic, 100ul and 500ul type I collagen, and Matrigel, or embedded within type I collagen. Cell morphology, cell number, and total TCA-precipitable {sup 35}S-labelled proteins were monitored. Milk protein ({alpha}{sub s,1}-casein, lactoferrin (LF), {alpha}-lactalbumin, and {beta}-lactoglobulin) secretion and intracellular levels were determined by an ELISA assay.
- Research Organization:
- Ohio State Univ., Columbus, OH (USA)
- OSTI ID:
- 5045196
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
550301 -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOASSAY
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BODY
BODY FLUIDS
CATTLE
CELL DIFFERENTIATION
CHEMICAL COMPOSITION
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
ENZYME IMMUNOASSAY
EVEN-ODD NUCLEI
FOOD
GLANDS
IMMUNOASSAY
IN VITRO
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MAMMARY GLANDS
MATERIALS
MILK
NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
RUMINANTS
SECRETION
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES
550301 -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOASSAY
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BODY
BODY FLUIDS
CATTLE
CELL DIFFERENTIATION
CHEMICAL COMPOSITION
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
ENZYME IMMUNOASSAY
EVEN-ODD NUCLEI
FOOD
GLANDS
IMMUNOASSAY
IN VITRO
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MAMMARY GLANDS
MATERIALS
MILK
NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
RUMINANTS
SECRETION
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES