Capillary permeability of /sup 131/I-albumin in skeletal muscle

Journal Article · Wed May 01 04:00:00 EDT 1985 · Microvasc. Res.; (United States)

DOI:https://doi.org/10.1016/0026-2862(85)90018-4· OSTI ID:5027917

Sejrsen, P; Paaske, W P; Henriksen, O

Capillary permeability of /sup 131/I-albumin in skeletal muscle was determined by applying the ''single injection, residue detection'' method to the autoperfused cat gastrocnemius muscle preparation. Experiments at zero venous pressure gave an average capillary extraction fraction, E, of 0.028 at a plasma flow of about 3 ml X (100 g)-1 X min-1. The permeability-surface area product, PS, was on average 0.075 ml X (100 g)-1 X min-1. Experiments at a venous pressure of 10 mm Hg gave essentially the same PS product (0.070 ml X (100 g)-1 X min-1. Further experiments demonstrated that stagnant intravascular pools of /sup 131/I-albumin were not present. The free diffusion coefficient in water at 37 degrees of the purified /sup 131/I-albumin was 0.0631 X 10-5 cm2 X sec-1). The distribution volume for /sup 131/I-albumin in the interstitial space was 3.5 ml X (100 g)-1 X sec-1. Assuming a capillary surface area of 70 cm2 X g-1 the permeability coefficient was calculated to be 18 X 10(-8) cm X sec-1 which is about 3 to 10 times higher than values obtained by lymph sampling methods. The results indicate that the predominant transcapillary transport mechanism for /sup 131/I-albumin is diffusion. When analyzed according to the theory of restricted diffusion the results are compatible with transcapillary diffusion through pores with an effective equivalent pore radius of 145 A.

• OSTI ID: 5027917
• Journal Information: Microvasc. Res.; (United States), Journal Name: Microvasc. Res.; (United States) Vol. 3; ISSN MIVRA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BLOOD VESSELS
BODY
CAPILLARIES
CAPILLARY FLOW
CARDIOVASCULAR SYSTEM
CATS
DAYS LIVING RADIOISOTOPES
DIFFUSION
FLUID FLOW
INTERMEDIATE MASS NUCLEI
IODINE 131
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
MEMBRANE TRANSPORT
MUSCLES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PERFUSED TISSUES
PERMEABILITY
PROTEINS
RADIOISOTOPES
TISSUES
TRACER TECHNIQUES
VERTEBRATES