Effects of ozone on lamb tracheal mucosa. Quantitative glycoconjugate histochemistry

Journal Article · Wed Nov 01 04:00:00 EST 1989 · American Journal of Pathology; (USA)

OSTI ID:5024383

Mariassy, A T; Sielczak, M W; McCray, M N; Abraham, W M; Wanner, A ^[1]

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1. Univ. of Miami, Miami Beach, FL (USA)

Whether or not the previously reported O3-induced abnormality in the postnatal development of tracheal secretory function in lambs is accompanied by changes in epithelial cell populations and their glycoconjugate composition was determined. Six lambs were killed at birth and 12 lambs at age 2 weeks. Of the latter 12, six were exposed to O3 (1 ppm, 4 hours daily for 5 days during the 1st week of life) and five had air-sham exposures (controls). Tracheal glycoconjugates were localized in situ with lectins to detect N-acetyl-galactosamine (galNAc), alpha-D-galactose (alpha-gal), beta-D-gal(1----3)-galNAc (beta-gal), and fucose (fuc). Mean (+/- SD) epithelial cell density (cells/mm basal lamina) was 418 +/- 57 in the newborns, 385 +/- 63 in controls (P was not significant), and 342 +/- 47 in O3-exposed lambs (P less than 0.05). Mucous cell density was 87 +/- 12 in newborns, 63 +/- 10 in controls (P less than 0.05), and 76 +/- 10 in O3 exposed lambs (P was not significant). Ciliated cells remained unchanged from birth to 2 weeks (P was not significant), but decreased (P less than 0.05) in O3-exposed lambs. All counted mucous cells contained fuc and galNAc at birth and retained these residues after sham and O3 exposure. The alpha-gal-containing mucous cells declined from 97 +/- 13 to 7 +/- 1 (P less than 0.05) and beta-gal containing cells from 39 +/- 5 to 25 +/- 4 in controls. In contrast, cells containing alpha-gal 71 +/- 10 remained at newborn levels (97 +/- 13) and beta-gal-containing cells increased from 40 +/- 5 at birth to 58 +/- 8 in O3-exposed animals (P less than 0.05). It was concluded that early postnatal exposure of lambs to O3 causes a decrease in epithelial cell density, but retards the developmental decrease in the number of tracheal mucous cells and alters the lectin detectable carbohydrate composition of mucus in these cells.

• OSTI ID: 5024383
• Journal Information: American Journal of Pathology; (USA), Journal Name: American Journal of Pathology; (USA) Vol. 135:5; ISSN AJPAA; ISSN 0002-9440
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALDEHYDES
ANIMAL TISSUES
ANIMALS
BODY
CARBOHYDRATES
DOMESTIC ANIMALS
EPITHELIUM
GALACTOSE
GLYCOPROTEINS
HEXOSES
LECTINS
MAMMALS
MEMBRANES
MONOSACCHARIDES
MUCOUS MEMBRANES
NEONATES
ORGANIC COMPOUNDS
OZONE
PATHOLOGICAL CHANGES
POLYSACCHARIDES
PROTEINS
RESPIRATORY SYSTEM
RUMINANTS
SACCHARIDES
SHEEP
TISSUES
TOXICITY
TRACHEA
VERTEBRATES