Inhibition of phosphorylation of cardiac troponin-I by adenosine and 5'-chloro 5'-deoxyadenosine
Conference
·
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:5022890
Adenosine inhibits the positive inotropic and chronotropic effects of catecholamines on the heart. Incubation of a crude contractile protein fraction from rat heart with (..gamma..-/sup 32/P)ATP results in the phosphorylation of several proteins by endogenous protein kinases. The adenosine analog 5'-chloro 5'-deoxyadenosine inhibits the phosphorylation of a 29 kDa protein in this preparation. The protein has been identified as cardiac troponin-I (cTn-I) by 2 dimensional gel electrophoresis, with a non-equilibrium pH gradient in the first direction and SDS-PAGE in the second, using purified cTn-I as standard. Addition of catalytic subunit of cAMP-dependent protein kinase to the crude contractile fraction increases phosphorylation of cTn-I; this increase is inhibited by 5'-chloro 5'-deoxyadenosine and adenosine. Phosphorylation of purified cTn-I by the catalytic subunit is also inhibited by 5'-chloro 5'-deoxyadenosine. Fifty percent inhibition of phosphorylation by either endogenous or exogenous kinase was observed at approximately 50 ..mu..M 5'-chloro 5'-deoxyadenosine or adenosine, a concentration within the intracellular range during hypoxia.
- Research Organization:
- Brandeis Univ., Waltham, MA
- OSTI ID:
- 5022890
- Report Number(s):
- CONF-8606151-
- Conference Information:
- Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADENOSINE
ATP
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
ELECTROPHORESIS
ENZYMES
INHIBITION
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHORYLATION
PHOSPHOTRANSFERASES
PROTEINS
RADIOISOTOPES
RIBOSIDES
TRACER TECHNIQUES
TRANSFERASES
59 BASIC BIOLOGICAL SCIENCES
ADENOSINE
ATP
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
ELECTROPHORESIS
ENZYMES
INHIBITION
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHORYLATION
PHOSPHOTRANSFERASES
PROTEINS
RADIOISOTOPES
RIBOSIDES
TRACER TECHNIQUES
TRANSFERASES