/sup 14/CO/sub 2/ production is no adequate measure of (/sup 14/C)fatty acid oxidation

Journal Article · Sun Jun 01 04:00:00 EDT 1986 · Biochem. Med. Metab. Biol.; (United States)

DOI:https://doi.org/10.1016/0885-4505(86)90080-0· OSTI ID:5021130

Veerkamp, J H; van Moerkerk, T B; Glatz, J F; Zuurveld, J G; Jacobs, A E; Wagenmakers, A J

Palmitate oxidation was comparatively assayed in various cell-free and cellular systems by /sup 14/CO/sub 2/ production and by the sum of /sup 14/CO/sub 2/ and /sup 14/C-labeled acid-soluble products. The /sup 14/CO/sub 2/ production rate was dependent on incubation time and amount of tissue in contrast to the total oxidation rate. The /sup 14/CO/sub 2/ contribution to the oxidation rate of (1-/sup 14/C)palmitate varied with homogenates from 1% with rat liver to 28% with rat kidney and amounted to only 2-4% with human muscles. With cellular systems the /sup 14/CO/sub 2/ contribution varied between 20% in human fibroblasts and 70% in rat muscles and myocytes. Addition of cofactors increased the oxidation rate, but decreased the /sup 14/CO/sub 2/ contribution. Various conditions appeared also to influence to a different extent the /sup 14/CO/sub 2/ production and the total oxidation rate with rat tissue homogenates and with rat muscle mitochondria. Incorporation of radioactivity from (1-/sup 14/C)palmitate into protein was not detectable in cell-free systems and only 2-3% of the sum of /sup 14/CO/sub 2/ and /sup 14/C-labeled acid-soluble products in cellular systems. Assay of /sup 14/CO/sub 2/ and /sup 14/C-labeled acid-soluble products is a much more accurate and sensitive estimation of fatty acid oxidation than assay of only /sup 14/CO/sub 2/.

• OSTI ID: 5021130
• Journal Information: Biochem. Med. Metab. Biol.; (United States), Journal Name: Biochem. Med. Metab. Biol.; (United States) Vol. 3; ISSN BMMBE
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BODY
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
CHALCOGENIDES
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
DIGESTIVE SYSTEM
FASTING
FIBROBLASTS
GLANDS
HEART
HEXADECANOIC ACID
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIVER
MAMMALS
MONOCARBOXYLIC ACIDS
MUSCLES
MYOCARDIUM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXIDATION
OXIDES
OXYGEN COMPOUNDS
RATS
RODENTS
SOMATIC CELLS
TRACER TECHNIQUES
VERTEBRATES