Labeled oxidation products from (1-/sup 14/C), (U-/sup 14/C) and (16-/sup 14/C)-palmitate in hepatocytes and mitochondria

Journal Article · Tue Sep 01 00:00:00 EDT 1987 · Lipids; (United States)

OSTI ID:5784940

Chatzidakis, C; Otto, D A

When (1-/sup 14/C), (U-/sup 14/C), and (16-/sup 14/C)palmitate were oxidized by isolated rat hepatocytes, there was a differential distribution of label as a percent of total oxidized products, such that /sup 14/CO/sub 2/ from (1-/sup 14/C) greater than (U-/sup 14/C) greater than (16-/sup 14/C)palmitate and acid-soluble radioactivity from (16-/sup 14/C) greater than (U-/sup 14/C) greater than (1-/sup 14/C)palmitate. The oxidation of (2,3-/sup 14/C)succinate to /sup 14/CO/sub 2/ by isolated hepatocytes was only 9.1% of that from (1,4-/sup 14/C)succinate, demonstrating that the differences in distribution of labeled products are in part due to less /sup 14/CO/sub 2/ production from label in the even carbon positions entering the citric acid cycle. Apparent total ketone body production from (16-/sup 14/C)palmitate was markedly higher than (1-/sup 14/C) and (U-/sup 14/C)palmitate. In addition, the /sup 14/C-acetone:/sup 14/CO/sub 2/ ratio derived from decarboxylation of labeled acetoacetate from (1-/sup 14/C)palmitate was less than 1 and positively correlated to the rate of fatty acid oxidation in hepatocytes. These findings indicate that the known preferential incorporation of the omega-C2 unit of fatty acids into /sup 14/C-ketone bodies also contributed to the differential distribution of labeled products and that this contribution was greatest at the lower rates of fatty acid oxidation. In isolated mitochondria, the distribution of label to /sup 14/CO/sub 2/ and acid-soluble radioactivity from (1-/sup 14/C), (U-/sup 14/C) and (16-/sup 14/C)palmitate was qualitatively similar to that seen with hepatocytes.

• Research Organization: Rutgers, State Univ. of New Jersey, New Brunswick
• OSTI ID: 5784940
• Journal Information: Lipids; (United States), Journal Name: Lipids; (United States) Vol. 22:9; ISSN LPDSA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACETONE
ANIMAL CELLS
ANIMALS
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL CULTURES
CHALCOGENIDES
HEXADECANOIC ACID
ISOTOPE APPLICATIONS
ISOTOPE DILUTION
KETONES
LABELLED COMPOUNDS
LIVER CELLS
MAMMALS
METABOLISM
MITOCHONDRIA
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
OXIDES
OXYGEN COMPOUNDS
RATS
RODENTS
SOMATIC CELLS
TRACER TECHNIQUES
VERTEBRATES