Membrane lipid dynamics in human promyelocytic leukemia cells sensitive and resistant to 12-O-tetradecanoylphorbol-13-acetate induction of differentiation

Journal Article · Sat Dec 01 04:00:00 EST 1984 · Cancer Res.; (United States)

OSTI ID:5019141

Fisher, P B; Schachter, D; Abbott, R E; Callaham, M F; Huberman, E

A series of fluorescent probes was used to analyze membrane lipid dynamics in promyelocytic leukemic cells sensitive (HL-60) or resistant (R-55) to phorbol diester induction of cell differentiation. When examined with the probe 1,6-diphenyl-1,3,5-hexatriene, R-55 cells were found to have higher fluorescence anisotropy values, indicative of decreased lipid fluidity, as compared to HL-60 cells. In contrast, when HL-60 and R-55 cells were compared using a series of membrane-impermeant fluorophores that incorporate only into the outer hemileaflet of the plasma membrane, no difference was observed in membrane lipid fluidity. Exposure to 12-O-tetradecanoylphorbol-13-acetate (10 ng/ml) for 24 hr decreased the fluorescence anisotropy of 1,6-diphenyl-1,3,5-hexatriene in both HL-60 and R-55 cells, whereas by 48 hr only the HL-60 cells displayed the reduction. No effect on the fluorescence anisotropy of 1-(4'-trimethylammonium phenyl)-6-phenyl-1,3,5-hexatriene, which is believed to localize in the plasma membrane, was observed in R-55 cells exposed to 12-O-tetradecanoylphorbol-13-acetate (10 or 100 ng/ml), whereas HL-60 cells treated with 12-O-tetradecanoylphorbol-13-acetate (10 ng/ml) showed a marked reduction in the fluorescence anisotropy. These observations suggest that the ability of HL-60 cells to respond to 12-O-tetradecanoylphorbol-13-acetate may be affected by the physical state of the plasma membrane lipids and that the resistant phenotype is associated with decreased fluidity of either the inner leaflet of the plasma membrane and/or of the cytosolic organellar membranes.

• Research Organization: Columbia Univ., New York, NY
• DOE Contract Number: W-31109-ENG-38
• OSTI ID: 5019141
• Journal Information: Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 44; ISSN CNREA
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANISOTROPY
BIOLOGICAL EFFECTS
CARCINOGENS
CELL CONSTITUENTS
CELL DIFFERENTIATION
CELL MEMBRANES
CONFORMATIONAL CHANGES
DISEASES
ESTERS
FLUORESCENCE
HEMIC DISEASES
LEUKEMIA
LIPIDS
LUMINESCENCE
MEMBRANES
NEOPLASMS
ORGANIC COMPOUNDS
PHORBOL ESTERS
TUMOR CELLS