Changes in DNA content of rat tracheal epithelial cells during neoplastic progression in vitro

Journal Article · Fri Jan 01 04:00:00 EST 1982 · Carcinogenesis (N.Y.); (United States)

DOI:https://doi.org/10.1093/carcin/3.8.847· OSTI ID:5018398

Braslawsky, G R; Flynn, K; Steele, V; Nettesheim, P

Flow cytometric DNA analysis was used to compare nuclear DNA content of carcinogen-induced F-344 rat tracheal epithelial cell lines as they progressed from non-tumorigenic (preneoplastic) to tumorigenic (neoplastic) populations in vitro. All of the tracheal epithelial cell lines established from carcinogen-treated tracheas showed increases in nuclear DNA content as compared to normal cell populations. For five cell lines, measurements were made during the preneoplastic state as well as after conversion to the neoplastic state. Four of the five cell lines showed a major shift in DNA content as the culture progressed from preneoplastic to neoplastic populations. However, there was no consistent change in DNA content as cultures progressed to neoplastic populations in vitro. Two cell lines showed shifts to higher levels as the cultures became tumorigenic, while two showed shifts to lower levels. Additionally two cell lines (3F3 and 165D) had DNA distribution profiles indicative of mixed cell populations during the neoplastic phase. These studies demonstrate that the transition from preneoplastic tracheal epithelial cells to neoplastic populations is often associated with a change in DNA content, and would suggest that the malignant cell type emerges as a new cell type from preneoplastic progenitor populations.

• Research Organization: Oak Ridge National Lab., TN
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 5018398
• Journal Information: Carcinogenesis (N.Y.); (United States), Journal Name: Carcinogenesis (N.Y.); (United States) Vol. 3:8; ISSN CRNGD
• Country of Publication: United States
• Language: English

Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AROMATICS
BIOLOGICAL EFFECTS
BODY
CARCINOGENESIS
CARCINOGENS
CELL FLOW SYSTEMS
CELL TRANSFORMATIONS
CONDENSED AROMATICS
DATA
DIMETHYLBENZANTHRACENE
DNA
EPITHELIUM
ESTERS
EXPERIMENTAL DATA
IN VITRO
INFORMATION
MAMMALS
NITROSO COMPOUNDS
NUCLEIC ACIDS
NUMERICAL DATA
ONCOGENIC TRANSFORMATIONS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PATHOGENESIS
PHORBOL ESTERS
RATS
RESPIRATORY SYSTEM
RODENTS
TISSUES
TRACHEA
TUMOR CELLS
VERTEBRATES