Recovery of hemopoietic stromal progenitor cells after lethal total-body irradiation and bone marrow transplantation in mice

Journal Article · Thu Aug 01 04:00:00 EDT 1985 · Transplantation; (United States)

DOI:https://doi.org/10.1097/00007890-198508000-00018· OSTI ID:5015843

Piersma, A H; Brockbank, K G; Ploemacher, R E; Ottenheim, C P

The recovery of fibroblastic colony-forming units (CFU-F) in murine bone marrow hemopoietic stroma was studied during eighteen months after 9 Gy lethal total-body irradiation and reconstitution with syngeneic bone marrow cells. After an initial depletion, CFU-F numbers increased from 10% of normal values at three months to 40% at 18 months after treatment, irrespective of graft size and presence of CFU-F in the graft. Fourteen months after treatment 35% of all CFU-F present in the recipients bone marrow was donor-derived independent of graft size. When mice were treated with high-dose lipopolysaccharide-W three months after irradiation and bone marrow transplantation, CFU-F numbers decreased to hardly detectable levels within one day, and then recovered to normal numbers four weeks later--whereas radiation control mice still had low CFU-F numbers. These data suggest that after lethal total-body irradiation the stroma still contained viable fibroblastic cells that had lost their in vitro colony-forming capacity as a result of radiation damage. In consequence there was no need for replacement of these fibroblastic cells by donor-derived or host-derived CFU-F. Only depletion of CFU-F from the bone marrow, as was induced with lipopolysaccharide, stimulated repopulation of the stroma with colony-forming fibroblastic cells.

• Research Organization: Erasmus Univ., Rotterdam, Netherland
• OSTI ID: 5015843
• Journal Information: Transplantation; (United States), Journal Name: Transplantation; (United States) Vol. 2; ISSN TRPLA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BODY
BONE MARROW
CARBOHYDRATES
COLONY FORMATION
CONNECTIVE TISSUE CELLS
EXTERNAL IRRADIATION
FIBROBLASTS
GRAFT-HOST REACTION
HEMATOPOIETIC SYSTEM
IRRADIATION
LETHAL IRRADIATION
LIPIDS
LIPOPOLYSACCHARIDES
MAMMALS
MICE
ORGANIC COMPOUNDS
ORGANS
POLYSACCHARIDES
RECOVERY
RODENTS
SACCHARIDES
SOMATIC CELLS
TISSUES
TRANSPLANTS
VERTEBRATES
VIABILITY
WHOLE-BODY IRRADIATION