Transcription factor OTF-1 interacts with two distinct DNA elements in the sup A. gamma. -globin gene promoter

Journal Article · Tue Mar 19 00:00:00 EST 1991 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00225a033· OSTI ID:5015122

Ponce, E; Lloyd, J A; Lingrel, J B ^[1]; Pierani, A; Roeder, R G ^[2]

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1. Univ. of Cincinnati College of Medicine, OH (United States)
2. Rockefeller Univ., New York, NY (United States)

A DNA region (site II) in the promoter of the human {sup A}{gamma}-globin gene ({minus}182 to {minus}168) is involved in transcriptional regulation. At least two nuclear proteins bind to this region: the erythroid-specific factor NF-E1/FG-1 and another factor present in many cell lines. In the present study, the authors demonstrate that the ubiquitous factor binding to site II has immunological identity with the octamer transcription factor OTF-1, which has been implicated in the regulation of expression of genes such as histone H2b and small nuclear RNA. In addition, they show that ORF-1 binds to site I ({minus}291 to {minus}267), a purine-rich region upstream of site II. Interestingly, OTF-1 binds to sites I and II with equal affinity. This was unexpected since the 14 bp site I binding site AAGAATAAATTAGA ({minus}291 to {minus}278), determined by methylation interference, does not show obvious similarities to the canonical octamer binding site for OTF-1 in site II (ATGCAAAT). Interaction of OTF-1 with functionally active binding sites in the {gamma}-globin promoter suggests that this factor has a role in {gamma}-globin transcription.

• OSTI ID: 5015122
• Journal Information: Biochemistry; (United States), Vol. 30:11; ISSN 0006-2960
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DNA
CROSS-LINKING
GLOBINS
GENE REGULATION
TRANSCRIPTION FACTORS
GENE REPRESSORS
HELA CELLS
HEPATOMAS
METHYLATION
MICE
PURINES
TUMOR CELLS
ANIMAL CELLS
ANIMALS
AROMATICS
AZAARENES
CHEMICAL REACTIONS
DISEASES
HETEROCYCLIC COMPOUNDS
MAMMALS
NEOPLASMS
NUCLEIC ACIDS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
POLYMERIZATION
PROTEINS
RODENTS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques