Isolation and amino acid sequences of squirrel monkey (Saimiri sciurea) insulin and glucagon

Journal Article · Fri Nov 30 23:00:00 EST 1990 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.87.24.9766· OSTI ID:5012953

Yu, Jinghua ^[1]; Eng, J; Yalow, R S ^[2]

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1. Veterans Administration Medical Center, Bronx, NY (United States)
2. Veterans Administration Medical Center, Bronx, NY (United States) City Univ. of New York, NY (United States)

It was reported two decades ago that insulin was not detectable in the glucose-stimulated state in Saimiri sciurea, the New World squirrel monkey, by a radioimmunoassay system developed with guinea pig anti-pork insulin antibody and labeled park insulin. With the same system, reasonable levels were observed in rhesus monkeys and chimpanzees. This suggested that New World monkeys, like the New World hystricomorph rodents such as the guinea pig and the coypu, might have insulins whose sequences differ markedly from those of Old World mammals. In this report the authors describe the purification and amino acid sequences of squirrel monkey insulin and glucagon. They demonstrate that the substitutions at B29, B27, A2, A4, and A17 of squirrel monkey insulin are identical with those previously found in another New World primate, the owl monkey (Aotus trivirgatus). The immunologic cross-reactivity of this insulin in their immunoassay system is only a few percent of that of human insulin. It appears that the peptides of the New World monkeys have diverged less from those of the Old World mammals than have those of the New World hystricomorph rodents. The striking improvements in peptide purification and sequencing have the potential for adding new information concerning the evolutionary divergence of species.

• OSTI ID: 5012953
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 87:24; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINO ACID SEQUENCE
ANIMALS
BIOASSAY
BODY
CHROMATOGRAPHY
DIABETES MELLITUS
DIAGNOSTIC TECHNIQUES
DIGESTIVE SYSTEM
DISEASES
ENDOCRINE DISEASES
ENDOCRINE GLANDS
GLANDS
GLUCAGON
GUINEA PIGS
HORMONES
IMMUNOASSAY
IMMUNOLOGY
INSULIN
ISOTOPE APPLICATIONS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
METABOLIC DISEASES
MOLECULAR STRUCTURE
MONKEYS
ORGANIC COMPOUNDS
ORGANS
PANCREAS
PEPTIDE HORMONES
PEPTIDES
POLYPEPTIDES
PRIMATES
PROTEINS
PURIFICATION
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
RODENTS
SEPARATION PROCESSES
TRACER TECHNIQUES
VERTEBRATES