Rapid in vitro drug-sensitivity assay in acute nonlymphocytic leukemia

Journal Article · Tue Nov 01 04:00:00 EST 1983 · Am. J. Hematol.; (United States)

DOI:https://doi.org/10.1002/ajh.2830150307· OSTI ID:5011667

Theodorakis, M E; Goldberg, J

Leukemic myeloid, myelomonocytic, and monocytic cells will incorporate radiolabeled sulfate into newly synthesized macromolecules. A liquid culture technique was developed to examine the in vitro effects of chemotherapeutic agents on the incorporation of radiolabeled sulfate into cells of patients with acute nonlymphocytic leukemia (ANLL). Cells recovered from bone marrow or peripheral blood of 25 patients with ANLL were incubated in vitro for one hour with saline (control) or a variety of chemotherapeutic agents. Cells were washed free of the drug and grown in liquid cultures containing nutrient medium and /sup 35/SO4. The percent of /sup 35/SO4 incorporated into the drug treated as compared with control cells was determined after one, three, and seven days of culture. Patients whose drug-treated cells incorporated less than 30% of /sup 35/SO4 when compared with the control after three or seven days of culture achieved a complete response to these agents in vivo. Thus, the in vitro effects of various chemotherapeutic agents on the incorporation of /sup 35/SO4 into cells obtained from patients with ANLL may help predict clinical response to these agents in vivo.

• Research Organization: State Univ. of New York, Syracuse
• OSTI ID: 5011667
• Journal Information: Am. J. Hematol.; (United States), Journal Name: Am. J. Hematol.; (United States) Vol. 15:3; ISSN AJHED
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CELL CULTURES
CHEMOTHERAPY
DAYS LIVING RADIOISOTOPES
DISEASES
EVALUATION
EVEN-ODD NUCLEI
FUNCTIONS
HEMIC DISEASES
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LEUKEMIA
LIGHT NUCLEI
MYELOID LEUKEMIA
NEOPLASMS
NUCLEI
PATIENTS
RADIOISOTOPES
REACTION KINETICS
RETENTION FUNCTIONS
SOMATIC CELLS
STEM CELLS
SULFUR 35
SULFUR COMPOUNDS
SULFUR ISOTOPES
THERAPY