A comparison of the rate of DNA synthesis in myeloblasts from peripheral blood and bone marrows of patients with acute nonlymphocytic leukemia

Journal Article · Sun May 01 04:00:00 EDT 1988 · Experimental Cell Research; (United States)

DOI:https://doi.org/10.1016/0014-4827(88)90116-4· OSTI ID:5451436

Raza, A; Yasin, Z; Grande, C ^[1]

---

1. Roswell Park Memorial Institute, Buffalo, NY (USA)

Durations of S-phase (T{sub s}) and total cell cycle times (T{sub c}) were measured from the peripheral blood (PB) and bone marrow aspirates (BM) of five patients with acute nonlymphocytic leukemia (ANLL). Intravenous bromodeoxyuridine (BrdU) was used as the first label for S-phase cells and a monoclonal anti-BrdU antibody was used to detect the positive cells. Tritiated thymidine (({sup 3}H)Tdr) was used as a second label in vitro, and the T{sub s} was calculated by counting the number of cells labeled either by BrdU or by ({sup 3}H)Tdr or by both. The data demonstrate that the duration of S-phase in myeloblasts obtained from BM is quite similar to that of circulating leukemic cells. Finally, the most accurate assessment of percentage of myeloblasts actively engaged in DNA synthesis can be obtained only from bone marrow biopsies following in vivo labeling.

• OSTI ID: 5451436
• Journal Information: Experimental Cell Research; (United States), Journal Name: Experimental Cell Research; (United States) Vol. 175:2; ISSN ECREA; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANTIMETABOLITES
AZINES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
BONE MARROW CELLS
BROMOURACILS
BUDR
CELL CYCLE
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
DISEASES
DNA REPLICATION
DRUGS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
IMMUNE SYSTEM DISEASES
LEUKEMIA
MATERIALS
NEOPLASMS
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RIBOSIDES
SOMATIC CELLS
THYMIDINE
TRITIUM COMPOUNDS
TUMOR CELLS
URACILS