Platelet antibody in idiopathic thrombocytopenic purpura and other thrombocytopenias
Platelet-associated immunoglobulin was measured by the use of fluorescent anti-1gG antibody. The method is simple, rapid, and sensitive and provides a precise quantitive assay of bound (direct) and free (indirect) 1gG with platelet specificity. We have evaluated this test in 30 normal volunteers and in 50 patients with immune and nonimmune, treated and untreated thrombocytopenias. All patients with immune thrombocytopenias (acute and chronic idiopathic thrombocytopenic purpura and systemic lupus erythematosus) having platelet counts < 100,000/..mu..l had elevated levels of platelet-bound 1gG and 86% had also positive results in the indirect assay. All patients with nonimmunological thrombocytopenias showed normal results in the direct and indirect assay of platelet-associated immunoglobulin. In patients studied repeatedly during the course of their illness, an inverse relation was found between platelet count and level of platelet-bound 1gG. Patients with systemic lupus erythematosus presented clear exceptions to this rule. Investigations of the absorbability of platelet autoantibodies and alloantibodies showed that this assay can readily differentiate between these two antibody species and can also identify specificities of alloantibodies.
- OSTI ID:
- 5008387
- Journal Information:
- J. Lab. Clin. Med.; (United States), Journal Name: J. Lab. Clin. Med.; (United States) Vol. 96:4; ISSN JLCMA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIBODIES
BIOASSAY
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY FLUIDS
CORRELATIONS
DATA
DISEASES
EXPERIMENTAL DATA
GLOBULINS
HEMIC DISEASES
IMMUNITY
IMMUNOGLOBULINS
INFECTIOUS DISEASES
INFORMATION
LUPUS
MAMMALS
MAN
MATERIALS
NUMERICAL DATA
ORGANIC COMPOUNDS
PATIENTS
PRIMATES
PROTEINS
PURPURA
SKIN DISEASES
SPECIFICITY
TUBERCULOSIS
VERTEBRATES