Effect of respiratory syncytial virus (RSV) infection on the adherence of pathogenic bacteria to human epithelial cells
The effect of RSV infection on the adherence of Streptococcus pneumoniae (SP), Haemophilus influenzae (HI) and Staphylococcus aureus (SA) to human epithelial cells was determined. RSV-infected Hep-2 cell cultures at different stages of expression of surface viral antigens and bacteria labeled with /sup 3/H-thymidine were employed to examine the kinetics of bacterial adherence to virus-infected cells. RSV infection did not alter the magnitude of adherence of HI or SA to HEp-2 cells. However, adherence of SP to HEp-2 cells was significantly (P < 0.01) enhanced by prior RSV infection. The degree of adherence was directly related to the amount of viral antigen expressed on the cell surface. The adherence was temperature dependent, with maximal adherence observed at 37/sup 0/C. Heat-inactivation of SP did not alter adherence characteristics. These data suggest that RSV infection increases adherence of SP to the surface of epithelial cells in vitro. Since attachment of bacteria to mucosal surfaces is the first step in many infections, it is suggested that viral infections of epithelial cells render them more susceptible to bacterial adherence. Thus, RSV infection in vivo may predispose children to SP infections, such as in otitis media, by increasing colonization with SP.
- Research Organization:
- State Univ. of New York, Buffalo
- OSTI ID:
- 5006886
- Report Number(s):
- CONF-8604222-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:3; ISSN FEPRA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ADHESION
ANIMAL TISSUES
AZINES
BACTERIA
BACTERIAL DISEASES
BIOLOGICAL EFFECTS
BODY
DISEASES
EPITHELIUM
HAEMOPHILUS
HETEROCYCLIC COMPOUNDS
INFECTIOUS DISEASES
INFECTIVITY
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MICROORGANISMS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PATHOGENESIS
PATHOGENS
PYRIMIDINES
RESPIRATORY SYSTEM
RIBOSIDES
STAPHYLOCOCCUS
STREPTOCOCCUS
THYMIDINE
TISSUES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VIRAL DISEASES