Reduced in vitro immune responses of purified human Leu-3 (helper/inducer phenotype) cells after total lymphoid irradiation

Journal Article · Wed Feb 01 04:00:00 EST 1984 · J. Immunol.; (United States)

OSTI ID:5005209

Field, E H; Engleman, E G; Terrell, C P; Strober, S

Patients treated with total lymphoid irradiation (TLI) for intractible rheumatoid arthritis showed marked decreases in the in vitro proliferative responses of peripheral blood mononuclear cells (PBM) to antigens and mitogens. To determine whether an intrinsic deficit in helper/inducer cell proliferation contributed to decreased responses, cells of the helper/inducer phenotype were purified from the PBM of treated patients by using monoclonal anti-Leu-3 antibody and a modified panning procedure. The purified Leu-3 cells obtained after TLI showed a marked reduction in (/sup 3/H)thymidine incorporation in response to allogeneic lymphocytes, PHA, Con A, and several protein antigens, as compared with that of cells from the same patients obtained before TLI. In addition, the quantity of Leu-3 surface antigen on the panned cells was reduced after TLI. The results suggest that TLI induces prolonged qualitative as well as quantitative changes in circulating Leu-3 T cells. These changes may contribute to the clinical effects of TLI.

• Research Organization: Stanford Univ. School of Medicine, CA
• OSTI ID: 5005209
• Journal Information: J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 132:2; ISSN JOIMA
• Country of Publication: United States
• Language: English

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62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DISEASES
EXTERNAL IRRADIATION
HETEROCYCLIC COMPOUNDS
IMMUNE REACTIONS
IN VITRO
IRRADIATION
LABELLED COMPOUNDS
LEUKOCYTES
LYMPHATIC SYSTEM
LYMPHOCYTES
MATERIALS
MEDICINE
NUCLEAR MEDICINE
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARTIAL BODY IRRADIATION
PATIENTS
PYRIMIDINES
RADIATION EFFECTS
RADIOLOGY
RADIOTHERAPY
RHEUMATIC DISEASES
RIBOSIDES
SIDE EFFECTS
SKELETAL DISEASES
SOMATIC CELLS
THERAPY
THYMIDINE
TRITIUM COMPOUNDS