Heterogeneity within the spleen colony-forming cell population in rat bone marrow
The pluripotent hemopoietic stem cell (HSC) of the rat can be enumerated in a spleen colony assay (SCA) in rats as well as mice. After injection of rat bone marrow into lethally irradiated mice, macroscopically visible spleen colonies (CFU-S) are found from day 6 through 14, but the number varies on consecutive days. In normal bone marrow a constant ratio of day-8 to day-12 colony numbers is observed. However, this ratio is changed after in vivo treatment of rats with cyclophosphamide, as well as after in vitro treatment of rat bone marrow with cyclophosphamide derivatives. This indicates that the CFU-S that form colonies on day 8 react differently to this treatment than the CFU-S that form colonies on day 12, and suggests heterogeneity among the CFU-S population. Posttreatment regrowth of day-8 and day-12 CFU-S is characterized by differences in population-doubling times (Td = 0.85 days vs 1.65 days). Another argument in support of the postulate of heterogeneity within the rat CFU-S population is derived from the fact that (in contrast to normal rat spleen) the spleen of leukemic rats contains high numbers of CFU-S that show a ratio of day-8 to day-12 CFU-S of 4.5, which is different than that observed for a CFU-S population in normal bone marrow (a ratio of 2.4). It is concluded that, in rat hemopoiesis, two populations of spleen colony-forming cells can be distinguished using the rat-to-mouse SCA. This indicates that mouse and rat hemopoiesis are comparable in this respect and that heterogeneity in the stem cell compartment is a general phenomenon.
- Research Organization:
- Radiobiological Institute TNO, Rijswijk, The Netherlands
- OSTI ID:
- 5004682
- Journal Information:
- Exp. Hematol.; (United States), Vol. 8
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BONE MARROW
CFU
BIOLOGICAL RADIATION EFFECTS
BLOOD FORMATION
ENDOXAN
LETHAL IRRADIATION
MICE
RATS
RESPONSE MODIFYING FACTORS
SPLEEN
STEM CELLS
VARIATIONS
ALKYLATING AGENTS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BIOLOGICAL EFFECTS
BODY
DRUGS
HEMATOPOIETIC SYSTEM
IMMUNOSUPPRESSIVE DRUGS
IRRADIATION
MAMMALS
ORGANS
RADIATION EFFECTS
RODENTS
SOMATIC CELLS
TISSUES
VERTEBRATES
560152* - Radiation Effects on Animals- Animals