Enhanced iron removal from liver parenchymal cells in experimental iron overload: liposome encapsulation of HBED and phenobarbital administration

Journal Article · Fri Jul 01 04:00:00 EDT 1983 · Blood; (United States)

OSTI ID:5003484

Rahman, Y E; Cerny, E A; Lau, E H; Carnes, B A

The effectiveness of N,N'-bis(2-hydroxybenzyl)-ethylene-diamine-N,N'-diacetic acid (HBED) in removing radioiron introduced into the parenchymal cells of mouse liver as /sup 59/Fe-ferritin has been investigated. The effectiveness of HBED, an iron chelator of low water solubility, has also been compared with that of desferrioxamine (DF), an iron chelator of high water solubility and currently in clinical use for treatment of transfusional iron overload. Using the /sup 59/Fe excretion as the measure of effectiveness of chelation therapy and a standardized single chelator dose of 25 mg/kg, they have found that: (1) a saline suspension of HBED, prepared by sonication and given intraperitoneally to mice, promotes a small but significant increase in excretion of radioiron compared to the untreated controls, whereas DF, in its free form, is ineffective; (2) HBED encapsulated in lipid bilayers of liposomes and given intravenously is superior to nonencapsulated HBED; (3) DF encapsulated in small unilamellar liposomes is ineffective in removing iron given in the form of ferritin; (4) administration of phenobarbital in drinking water, at a concentration of 1 g/liter, induces a 30%-55% increase of iron excretion from untreated control mice and also from mice given HBED either in liposome-encapsulated or nonencapsulated form. HBED is superior to DF for removal of storage iron from liver parenchymal cells and liposomes are useful carriers for iron chelators of low water solubility.

• Research Organization: Argonne National Lab., IL
• OSTI ID: 5003484
• Journal Information: Blood; (United States), Journal Name: Blood; (United States) Vol. 62:1; ISSN BLOOA
• Country of Publication: United States
• Language: English

Related Subjects

560172* -- Radiation Effects-- Nuclide Kinetics & Toxicology-- Animals-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
AMINO ACIDS
ANESTHETICS
ANIMAL CELLS
ANIMALS
ANTICONVULSANTS
AZINES
BARBITURATES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CHELATING AGENTS
CLEARANCE
COMPLEXES
DAYS LIVING RADIOISOTOPES
DEFEROXAMINE
DIGESTIVE SYSTEM
DRUGS
EVEN-ODD NUCLEI
EXCRETION
FECES
FERRITIN
GLANDS
HETEROCYCLIC COMPOUNDS
HYPNOTICS AND SEDATIVES
INTERMEDIATE MASS NUCLEI
IRON 59
IRON COMPLEXES
IRON ISOTOPES
ISOTOPES
LIPOSOMES
LIVER
MAMMALS
MATERIALS
METALLOPROTEINS
MICE
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANOIDS
ORGANS
PHENOBARBITAL
PROTEINS
PYRIMIDINES
RADIOISOTOPES
RODENTS
TRANSITION ELEMENT COMPLEXES
VERTEBRATES
WASTES