Metabolism and DNA binding of BaP in the presence of complex organic mixtures

Conference · Tue Apr 01 04:00:00 EST 1986

OSTI ID:5003325

Springer, D L; Dankovic, D A; Thomas, B L; Hopkins, K L; Bean, R E; Mahlum, D D

Previous studies have shown that the mutagenic, carcinogenic and DNA binding activities of benzo(a)pyrene (BaP) were decreased when BaP was coadministered with complex organic mixtures. We have continued these studies to identify the components of the mixture that were responsible for this effect. For this study a high-boiling coal liquid was separated into aliphatic, polycyclic aromatic hydrocarbon (PAH), nitrogen-containing polycyclic aromatic compounds (NPAC) and oxygen containing PAH fractions (HPAH); these were spiked with /sup 3/H-BaP, administered dermally to mice and DNA isolated twenty-four hours later. Binding of BaP, as estimated by radioactivity associated the DNA, decreased to 47, 55 and 66% of that for the same dose of BaP alone for the PAH, NPAC and HPAH fractions, respectively. In vitro studies with Aroclor-induced rat liver S9 demonstrated that the rate of metabolism of BaP decreased when it was coadministered with the mixtures but that the BaP metabolite profiles were similar for BaP alone and in the presence of the mixture. These data suggest that the differences in mutagenic, and presumable carcinogenic activities, for BaP, when administered with the complex mixture, were due to decreased rates of conversion of BaP to reactive metabolites. 15 refs., 2 figs., 3 tabs.

• Research Organization: Pacific Northwest Lab., Richland, WA (USA)
• DOE Contract Number: AC06-76RL01830
• OSTI ID: 5003325
• Report Number(s): PNL-SA-13221; CONF-861044-1; ON: DE87001656
• Country of Publication: United States
• Language: English

Related Subjects

550501 -- Metabolism-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADDUCTS
AROMATICS
AZAARENES
BENZOPYRENE
CHROMATOGRAPHY
COAL LIQUIDS
CONDENSED AROMATICS
DNA ADDUCTS
FLUIDS
HETEROCYCLIC COMPOUNDS
HETEROCYCLIC OXYGEN COMPOUNDS
HYDROCARBONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
LIQUIDS
METABOLIC ACTIVATION
METABOLITES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
POLYCYCLIC AROMATIC HYDROCARBONS
SEPARATION PROCESSES
TRACER TECHNIQUES
TRITIUM COMPOUNDS