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Manganese containing protein complex isolated from Photosystem II preparations of spinach

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:5002989
;
By using a ligand-receptor crosslinking method the authors have stabilized Mn associated with Photosystem II (PSII) in a protein complex with an apparent molecular weight of 75,000 and with 3-4 Mn per complex. To crosslink the proteins, purified 33 kDa protein (33) was modified to contain about 8 adducts of the heterobifunctional photoaffinity reagent N-succinimidyl-(4-azidophenyl-dithio)-propionate (SADP). The SADP-33 was reconstituted into PSII membranes which had been depleted of 33 by a 1M CaCl/sub 2/ wash and crosslinking was initiated by ultraviolet illumination. The crosslinked membranes were solubilized in lauryl sulfate (SDS) and the constituent proteins were identified by SDS polyacrylamide gel electrophoresis. Evidence which supports the hypothesis that the Mn associated with the crosslinked proteins has been retained at the site of the photosynthetic oxygen evolving system includes: 1, Scatchard analysis of (/sup 125/I)-33 binding to CaCl/sub 2/-washed PSII preparations revealed one tight binding site and several lower affinity sites; 2, reconstitution of O/sub 2/ evolving activity and binding to the tight site had the same concentration dependence on 33; 3, the SADP-33 was able to reconstitute O/sub 2/ evolution in CaCl/sub 2/ washed PSII membranes; 4, the percent of Mn retained by the crosslinked membranes after treatment with edetic acid (EDTA) was approximately equal to the percent reconstitution of O/sub 2/ evolving activity by SADP-33 before photoactivation of SADP.
Research Organization:
Univ. of Michigan, Ann Arbor
OSTI ID:
5002989
Report Number(s):
CONF-8606151-
Conference Information:
Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
Country of Publication:
United States
Language:
English

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Related Subjects

550201 -- Biochemistry-- Tracer Techniques
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AFFINITY
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CHEMICAL REACTIONS
CROSS-LINKING
DAYS LIVING RADIOISOTOPES
ELECTROMAGNETIC RADIATION
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
ELEMENTS
FOOD
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
KINETICS
LIGANDS
MANGANESE
MEMBRANE PROTEINS
MEMBRANES
METALS
MOLECULAR WEIGHT
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PHOTOSYNTHETIC MEMBRANES
PHOTOSYNTHETIC REACTION CENTERS
PLANTS
POLYMERIZATION
PROTEINS
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
SPINACH
TRANSITION ELEMENTS
ULTRAVIOLET RADIATION
VEGETABLES