In vitro hydrogen production by glucose dehydrogenase and hydrogenase

Journal Article · Mon Jul 01 00:00:00 EDT 1996 · Bio/Technology

DOI:https://doi.org/10.1038/nbt0796-872· OSTI ID:495272

Woodward, J; Mattingly, S M ^[1]; Danson, M ^[2]

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1. Oak Ridge National Lab., TN (United States)
2. Univ. of Bath (United Kingdom); and others

A new in vitro enzymatic pathway for the generation of molecular hydrogen from glucose has been demonstrated. The reaction is based on the oxidation of glucose by Thermoplasma acidophilum glucose dehydrogenase with the concomitant oxidation of NADPH by Pyrococcus furiosus hydrogenase. Stoichiometric yields of hydrogen were produced from glucose with the continuous recycling of cofactor. This simple system may provide a method for the biological production of hydrogen from renewable sources. In addition, the other product of this reaction, gluconic acid, is a high-value chemical commodity. 23 refs., 5 figs.

• DOE Contract Number: AC05-96OR22464
• OSTI ID: 495272
• Journal Information: Bio/Technology, Vol. 14, Issue 7; Other Information: PBD: Jul 1996
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
55 BIOLOGY AND MEDICINE
BASIC STUDIES
OXIDOREDUCTASES
ENZYME ACTIVITY
HYDROGENASES
GLUCOSE
OXIDATION
INTERSTITIAL HYDROGEN GENERATION
EVALUATION
OPTIMIZATION
BIOLOGICAL PATHWAYS
DESIGN
RENEWABLE ENERGY SOURCES
GLUCONIC ACID
PRODUCTION
GENES
GENE REGULATION
DNA SEQUENCING
DNA-CLONING
HYDROGEN
ORNL
BIOMASS
NADP
BACTERIA
STOICHIOMETRY