In vitro hydrogen production by glucose dehydrogenase and hydrogenase

Journal Article · Mon Jul 01 00:00:00 EDT 1996 · Bio/Technology

DOI:https://doi.org/10.1038/nbt0796-872· OSTI ID:495272

Woodward, J; Mattingly, S M ^[1]; Danson, M ^[2]

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1. Oak Ridge National Lab., TN (United States)
2. Univ. of Bath (United Kingdom); and others

A new in vitro enzymatic pathway for the generation of molecular hydrogen from glucose has been demonstrated. The reaction is based on the oxidation of glucose by Thermoplasma acidophilum glucose dehydrogenase with the concomitant oxidation of NADPH by Pyrococcus furiosus hydrogenase. Stoichiometric yields of hydrogen were produced from glucose with the continuous recycling of cofactor. This simple system may provide a method for the biological production of hydrogen from renewable sources. In addition, the other product of this reaction, gluconic acid, is a high-value chemical commodity. 23 refs., 5 figs.

• DOE Contract Number: AC05-96OR22464
• OSTI ID: 495272
• Journal Information: Bio/Technology, Journal Name: Bio/Technology Journal Issue: 7 Vol. 14; ISSN 0733-222X; ISSN BTCHDA
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
55 BIOLOGY AND MEDICINE
BASIC STUDIES
BACTERIA
BIOLOGICAL PATHWAYS
BIOMASS
DESIGN
DNA SEQUENCING
DNA-CLONING
ENZYME ACTIVITY
EVALUATION
GENE REGULATION
GENES
GLUCONIC ACID
GLUCOSE
HYDROGEN
HYDROGENASES
INTERSTITIAL HYDROGEN GENERATION
NADP
OPTIMIZATION
ORNL
OXIDATION
OXIDOREDUCTASES
PRODUCTION
RENEWABLE ENERGY SOURCES
STOICHIOMETRY