In vitro hydrogen production by glucose dehydrogenase and hydrogenase
- Oak Ridge National Lab., TN (United States)
A new in vitro enzymatic pathway for the generation of molecular hydrogen from glucose has been demonstrated. The reaction is based upon the oxidation of glucose by Thermoplasma acidophilum glucose dehydrogenase with the concomitant oxidation of NADPH by Pyrococcus furiosus hydrogenase. Stoichiometric yields of hydrogen were produced from glucose with continuous cofactor recycle. This simple system may provide a method for the biological production of hydrogen from renewable sources. In addition, the other product of this reaction, gluconic acid, is a high-value commodity chemical.
- Research Organization:
- National Renewable Energy Lab., Golden, CO (United States)
- DOE Contract Number:
- AC05-96OR22464
- OSTI ID:
- 447146
- Report Number(s):
- NREL/CP--430-21968-Vol.1; CONF-9605195--Vol.1; ON: DE97000053
- Country of Publication:
- United States
- Language:
- English
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