Composting oily sludges: Characterizing microflora using randomly amplified polymorphic DNA
- ANOX AB, Lund (Sweden)
- Lund Univ. (Sweden). Lab. of Food Hygiene and Technology
Laboratory-scale composts in which oily sludge was composted under mesophilic conditions with amendments such as peat, bark, and fresh or decomposed horse manure, were studied with respect to basic parameters such as oil degradation, respirometry, and bacterial numbers. Further, an attempt was made to characterize a part of the bacterial flora using randomly amplified polymorphic DNA (RAPD). The compost based on decomposed horse manure showed the greatest reduction of oil (85%). Comparison with a killed control indicated that microbial degradation actually had occurred. However, a substantial part of the oil was stabilized rather than totally broken down. Volatiles, on the contrary, accounted for a rather small percentage (5%) of the observed reduction. RAPD indicated that a selection had taken place and that the dominating microbial flora during the active degradation of oil were not the same as the ones dominating the different basic materials. The stabilized compost, on the other hand, had bacterial flora with similarities to the ones found in peat and bark.
- OSTI ID:
- 492241
- Report Number(s):
- CONF-950483-; ISBN 1-57477-006-3; TRN: 97:011911
- Resource Relation:
- Conference: 3. international in situ and on-site bioreclamation symposium, San Diego, CA (United States), 24-27 Apr 1995; Other Information: PBD: 1995; Related Information: Is Part Of Monitoring and verification of bioremediation; Hinchee, R.E. [ed.] [Battelle Memorial Inst., Columbus, OH (United States)]; Douglas, G.S. [ed.] [Arthur D. Little, Inc., Cambridge, MA (United States)]; Ong, S.K. [ed.] [Iowa State Univ., Ames, IA (United States)]; PB: 283 p.; Bioremediation, Volume 3(5)
- Country of Publication:
- United States
- Language:
- English
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