Genetic adaptation of bacteria to halogenated aliphatic compounds
- Univ. of Groningen (Netherlands)
The bacterial degradation and detoxification of chlorinated xenobiotic compounds requires the production of enzymes that are capable of recognizing and converting compounds which do not occur at significant concentrations in nature. We have studied the catabolic route of 1,2-dichloroethane as an example of a pathway for the conversion of such a synthetic compound. In strains of Xanthobacter and Ancylobacter that have been isolated on 1,2-dichloroethane, the first catabolic step is catalyzed by a hydrolytic haloalkane dehalogenase. The enzyme converts 1,2-dichloroethane to 2-chloroethanol but is also active with many other environmentally important haloalkanes such as methylchloride, methylbromide, 1,2-dibromoethane, epichlorohydrin, and 1,3-dichloropropene. Further degradation of 2-chloroethanol proceeds by oxidation to the carboxylic acid and dehalogenation to glycolate. The aldehyde dehydrogenase prevents toxicity of the reactive chloroacetaldehyde that is formed as an intermediate and is necessary for establishing a functional 2-chloroethanol degradative pathway in a strain that is not capable of growth on this compound. 27 refs., 3 figs., 1 tab.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 486409
- Report Number(s):
- CONF-9304308-; ISSN 0091-6765; TRN: 97:000705-0006
- Journal Information:
- Environmental Health Perspectives, Vol. 103, Issue Suppl.5; Conference: Biodegradation: its role in reducing toxicity and exposure to environmental contaminants conference, Research Triangle Park, NC (United States), 23-26 Apr 1993; Other Information: PBD: Jun 1995
- Country of Publication:
- United States
- Language:
- English
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