Involvement of a large plasmid in the degradation of 1,2-dichloroethane by Xanthobacter autotrophicus

Journal Article · Sat Jun 01 00:00:00 EDT 1991 · Applied and Environmental Microbiology; (United States)

OSTI ID:5412803

Tardif, G; Greer, C W; Labbe, D; Lau, P C.K. ^[1]

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1. National Research Council of Canada Biotechnology Research Inst., Montreal, Quebec (Canada)

Xanthobacter autotrophicus GJ10 is a bacterium that can degrade short-chain halogenated aliphatic compounds such as 1,2-dichloroethane. A 200-kb plasmid, pXAU1, was isolated from this strain and shown to contain the dhlA gene, which codes for haloalkane dehalogenase, the first enzyme in the degradation pathway of 1,2-dichloroethane by GJ10. Loss of pXAU1 resulted in loss of haloalkane dehalogenase activity, significantly decreased chloroacetaldehyde dehydrogenase activity, and loss of resistance to mercuric chloride but did not affect the activity level of haloalkanoate dehalogenase, the second dehalogenase in the degradation of 1,2-dichloroethane.

• OSTI ID: 5412803
• Journal Information: Applied and Environmental Microbiology; (United States), Vol. 57:6; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
CHLORINATED ALIPHATIC HYDROCARBONS
BIODEGRADATION
BACTERIA
BIOLOGICAL PATHWAYS
ENZYME ACTIVITY
GENES
HYDROLASES
MERCURY CHLORIDES
METABOLISM
OXIDOREDUCTASES
PLASMIDS
TOLERANCE
CELL CONSTITUENTS
CHEMICAL REACTIONS
CHLORIDES
CHLORINE COMPOUNDS
DECOMPOSITION
ENZYMES
HALIDES
HALOGEN COMPOUNDS
HALOGENATED ALIPHATIC HYDROCARBONS
MERCURY COMPOUNDS
MERCURY HALIDES
MICROORGANISMS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
540210* - Environment
Terrestrial- Basic Studies- (1990-)
540310 - Environment
Aquatic- Basic Studies- (1990-)