Reduction of azo dyes and nitroaromatic compounds by bacterial enzymes from the human intestinal tract
- Food and Drug Administration, Jefferson, AR (United States)
Several anaerobic bacteria from the human intestinal tract are capable of reducing azo dyes and nitropolycyclic aromatic hydrocarbons to the corresponding aromatic amines with enzymes that have azoreductase and nitroreductase activities. The majority of bacteria with these activities belong to the genera Clostridium and Eubacterium. The azoreductases and nitroreductases from three Clostridium strains and one Eubacterium strain were studied. Both enzymes were produced constitutively in each of the bacteria; the enzymes from various bacteria had different electrophoretic mobilities. The azoreductases from all of the bacteria had immunological homology, as was evident from the cross-reactivity of an antibody raised against the azoreductase of C perfringens with azoreductases from other bacteria. Comparison of azoreductases and nitroreductases showed that they both had identical electrophoretic mobilities on polyacrylamide gels and reacted with the antibody against the azoreductase from C. perfringens. Furthermore, the nitroaromatic compounds competitively inhibited the azoreductase activity. The data indicate that the reduction of both nitroaromatic compounds and azo dyes may be carried out by the same enzyme, which is possibly a flavin adenine dinucleotide dehydrogenase that is synthesized throughout the cell and not associated with any organized subcellular structure. 15 refs., 1 fig., 2 tabs.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 486407
- Report Number(s):
- CONF-9304308-; ISSN 0091-6765; TRN: 97:000705-0004
- Journal Information:
- Environmental Health Perspectives, Vol. 103, Issue Suppl.5; Conference: Biodegradation: its role in reducing toxicity and exposure to environmental contaminants conference, Research Triangle Park, NC (United States), 23-26 Apr 1993; Other Information: PBD: Jun 1995
- Country of Publication:
- United States
- Language:
- English
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