Microbial activity in subsurface samples before and during nitrate-enhanced bioremediation
- Rice Univ., McLean, VA (United States)
- Rice Univ., Houston, TX (United States). Energy and Environmental Systems Inst.
- Environmental Protection Agency, Ada, OK (United States). R.S. Kerr Environmental Research Lab.
A study was conducted to determine the microbial activity at a site contaminated with JP-4 jet fuel before and during nitrate-enhanced bioremediation. Samples at three depths from six different locations were collected aseptically under anaerobic conditions before and during treatment. Cores were located in or close to the source of contamination, downgradient of the source, or outside the zone of contamination. Parameters for microbial characterization included (1) viable counts of aerobic heterotrophic, JP-4 degrading, and oligotrophic bacteria; (2) the most probable number (MPN) of aerobic and anaerobic protozoa; (3) the MPN of total denitrifiers; and (4) the MPN of denitrifiers in hydrocarbon-amended microcosms. The results indicate that the total number of denitrifiers increased by an order of magnitude during nitrate-enhanced bioremediation in most samples. The number of total heterotrophs and JP-4-degrading microorganisms growing aerobically also increased. In addition, the first anaerobic protozoa associated with hydrocarbon-contaminated subsurface materials were detected.
- OSTI ID:
- 484970
- Report Number(s):
- CONF-950483-; ISBN 1-57477-009-8; TRN: 97:010834
- Resource Relation:
- Conference: 3. international in situ and on-site bioreclamation symposium, San Diego, CA (United States), 24-27 Apr 1995; Other Information: PBD: 1995; Related Information: Is Part Of Microbial processes for bioremediation; Hinchee, R.E.; Brockman, F.J. [eds.] [Battelle Memorial Inst., Columbus, OH (United States)]; Vogel, C.M. [ed.]; PB: 371 p.; Bioremediation, Volume 3(8)
- Country of Publication:
- United States
- Language:
- English
Similar Records
Laboratory and field studies on BTEX biodegradation in a fuel-contaminated aquifer under denitrifying conditions. Book chapter, May 88-Dec 92
Potential for Microbial Stimulation in Deep Vadose Zone Sediments by Gas-Phase Nutrients