Substrate range of the (chloro)biphenyl degradation pathway of Alcaligenes sp. JB1

Conference · Sun Dec 31 04:00:00 EST 1995

OSTI ID:484961

Parsons, J R; Goorissen, H; Weiland, A R; Bruijne, J.A. de ^[1]; Springael, D; Lelie, D van der; Mergeay, M ^[2]

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1. Univ. of Amsterdam (Netherlands). Dept. of Environmental and Toxicological Chemistry
2. VITO/Flemish Inst. for Technological Research, Mol (Belgium). Lab. of Genetics and Biotechnology

The genes encoding the biphenyl degradation pathway of Alcaligenes sp. strain JB1 were cloned in an R-prime plasmid in Alcaligenes eutrophus strain AE53. Subsequently, recombinant Alcaligenes eutrophus strains containing the bphABC genes of JB1 were selected by the accumulation of a yellow ring-cleaved product from biphenyl and 2,3-dihydroxy-biphenyl. Gluconate-grown cultures of one of these recombinants were tested for their ability to degrade 2,2{prime},3,3{prime}-tetrachlorobiphenyl, 2-chloro-dibenzo-p-dioxin, 1-chloronaphthalene, 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (p,p{prime}-DDT), and 2,4{prime}-dichlorodiphenyl ether. Depending on their solubility, the initial concentrations of these compounds were between 10 and 100 {micro}g/L. In all cases more than 65% of these compounds was removed within a 24-h incubation period. These results show that the enzymes responsible for degrading (chloro)biphenyls in strain JB1 are able to attack a wide range of aromatic compounds. This strain, or those containing similar enzymes, may be applicable to the bioremediation of mixtures of contaminants.

• OSTI ID: 484961
• Report Number(s): CONF-950483--; ISBN 1-57477-009-8
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
BACTERIA
BIODEGRADATION
BIOLOGICAL PATHWAYS
CHLORINATED AROMATIC HYDROCARBONS
DIOXIN
GENE REGULATION
METABOLISM
REMEDIAL ACTION
SUBSTRATES