Transcription elongation factor SII (TCEA) maps to human chromosome 3p22 {r_arrow} p21.3

Journal Article · Thu Aug 15 04:00:00 EDT 1996 · Genomics

DOI:https://doi.org/10.1006/geno.1996.0443· OSTI ID:484343

DiMarco, S P; Reines, D; Warren, S T ^[1]

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1. Emory Univ. School of Medicine, Atlanta, GA (United States); and others

Transcription elongation factors assist RNA polymerase II through transcriptional blockades. The human transcriptional elongation factor SII or Transcription Elongation Factor A (TCEA) releases RNA polymerase II from transcriptional arrest and is encoded by a 2.5-kb intronless gene. Using PCR primers, verified by RT-PCR to amplify the authentic, transcriptionally active SII gene, this locus was mapped to human chromosome 3 by examination of a humari/rodent somatic cell hybrid panel. PCR analysis of somatic cell hybrids with chromosome 3 translocations and FISH studies utilizing a human YAC clone containing the SII gene further refine the map position of this locus to human chromosome 3p22 {r_arrow} p21.3. Since another elongation factor, SIII, has been implicated in human carcinogenesis and since the interval within which the human SII gene maps is frequently deleted in certain cancers, elongation factor SII may therefore be considered a candidate gene for human malignancies involving 3p22 - p21.3. 29 refs., 2 figs.

• OSTI ID: 484343
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 1 Vol. 36; ISSN 0888-7543; ISSN GNMCEP
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
CHROMOSOMAL ABERRATIONS
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
FLUORESCENCE
GENE REGULATION
GENETIC MAPPING
HEREDITARY DISEASES
HUMAN CHROMOSOME 3
HYBRIDIZATION
MAN
NEOPLASMS
POLYMERASE CHAIN REACTION
RESOLUTION
RNA POLYMERASES
SOMATIC CELLS
TRANSCRIPTION
TRANSCRIPTION FACTORS