Isolation of a human YAC contig encompassing a cluster of UGT2 genes and its regional localization to chromosome 4q13
- Ninewells Hospital Medical School, Scotland (United Kingdom); and others
Previously the authors mapped the gene encoding a human bile acid UDP-glucuronosyltransferase (UGT2B4) to chromosome 4. Here they report the mapping of two additional human UGT2B genes to chromosome 4 utilizing the polymerase chain reaction (PCR) and a panel of human/rodent somatic cell hybrid cell lines. A yeast artificial chromosome contig containing the UGT2B4, UGT2B9, and UGT2B15 genes were isolated, and pulsed-field gel electrophoresis and PCR revealed that several members of the human UGT2B gene subfamily are clustered within a 195-kb region of the YAC contig. These data permitted a provisional ordering of the genes as UGT2B9-UGT2B4-UGT2B15. Fluorescence in situ hybridization analysis, using the YAC DNA, permitted the regional localization of this gene cluster to chromosome 4q13. 22 refs., 2 figs., 1 tab.
- OSTI ID:
- 255152
- Journal Information:
- Genomics, Journal Name: Genomics Journal Issue: 2 Vol. 23; ISSN GNMCEP; ISSN 0888-7543
- Country of Publication:
- United States
- Language:
- English
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